摘要
目的研究性别对成年C57BL/6小鼠心肌细胞收缩力和钙瞬变的影响。方法采用Langendorff逆行灌流、Ⅱ型胶原酶消化方法分离不同性别成年C57BL/6小鼠心肌细胞,比较两组心肌细胞的形态学差异;运用Ion Optix细胞收缩及离子浓度同步测量系统,同步记录不同性别小鼠心肌细胞肌节长度及钙瞬变随电刺激的变化,比较两组间心肌细胞收缩力(肌节缩短幅度、肌节缩短率、肌节最大收缩速度和最大舒张速度)和心肌细胞钙瞬变(钙瞬变峰值、钙瞬变幅度和钙离子消除时间常数)的差异。结果成年雄性C57BL/6小鼠心肌细胞收缩力指标:肌节缩短幅度[(0.14±0.02)μm比(0.08±0.01)μm]、肌节缩短率(7.47%±0.93%比4.60%±0.59%)、肌节最大收缩速度[(-3.25±0.37)μm/s比(-2.11±0.38)μm/s]和肌节最大舒张速度[(2.45±0.29)μm/s比(1.57±0.31)μm/s]明显高于雌性小鼠(均为P<0.05)。此外,成年雄性C57BL/6小鼠心肌细胞钙瞬变指标钙瞬变峰值和钙瞬变幅度亦显著高于雌性小鼠(均为P<0.05),而雄性小鼠心肌细胞钙离子消除时间常数低于雌性小鼠[(122.40±22.99)ms比(189.20±19.54)ms,P<0.05]。结论成年雄性C57BL/6小鼠的心肌细胞收缩力及钙瞬变均高于雌性小鼠。
Objective To explore the effect of gender on the contractility and calcium transient of adult C57BL/6 mouse cardiomyocytes. Methods Adult mouse cardiomyocytes were isolated by retrograde perfusion of collagenaseⅡenzymolysis with Langendorff perfusion system.The morphological differences of the two groups(male and female)of mouse cardiomyocytes were compared.Sarcomere length and calcium transient changes of both male and female under field stimulation were recorded synchronously with IonOptix Myocyte Calcium and Contractility System.Parameters that reflect cardiomyocyte contractility such as the sarcomere shortening amplitude,sarcomere shortening rate,the maximal systolic rate,the maximal diastolic rate,and those reflect calcium transient such as peak calcium transient,calcium transient amplitude,and calcium decay time constant were analyzed and compared between the two groups. Results The sarcomere length peak height[(0.14±0.02)μm vs.(0.08±0.01)μm],sarcomere shortening rate(7.47%±0.93%vs.4.60%±0.59%),the maximal systolic rate[(-3.25±0.37)μm/s vs.(-2.11±0.38)μm/s],and the maximal diastolic rate[(2.45±0.29)μm/s vs.(1.57±0.31)μm/s]of adult male C57BL/6 mice were apparently larger than that of female.The calcium transient peak and calcium transient amplitude were also much greater than female.Accordingly,the calcium decay time constant[(122.40±22.99)ms vs.(189.20±19.54)ms]of adult male C57BL/6 mouse cardiomyocytes was smaller compared with that of female. Conclusions Compared with the female mice,male C57/BL6 mice have greater cardiomyocyte contractility and calcium transient.
作者
牛小豆
李子健
张永珍
Niu Xiaodou;Li Zijian;Zhang Yongzhen(Department of Cardiology and Institute of Vascular Medicine,Peking University Third Hospital;Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides,Ministry of Health;Key Laboratory of Molecular Cardiovascular Science,Ministry of Education;Beijing Key Laboratory of Cardiovascu)
出处
《中国心血管杂志》
2018年第2期152-155,共4页
Chinese Journal of Cardiovascular Medicine
基金
国家重点基础研究发展规划项目(973)计划(2014CBA02000)
国家自然科学基金项目(81471893
91539123)~~
关键词
小鼠
肌细胞
心脏
性别
收缩力
钙瞬变
Mice
Myocytes,cardiac
Sex
Contractility
Calcium transient
