摘要
目的观察内皮素A受体拮抗剂预处理对犬体外循环(CPB)后肾功能影响。方法选择18只雄性比格犬,采用随机数字表法分为3组(n=6):假手术组(Sham组)、CPB组、内皮素A受体拮抗剂组(ETA组)。ETA组中,CPB前1h持续泵入内皮素A受体拮抗剂西他生坦0.7mg/kg,30min输注完毕。采集各组体温、平均动脉压及动脉血气,检测血肌酐、尿素氮浓度,评估肾小管坏死评分,并测定磷酸化蛋白激酶(pAkt),磷酸化内皮型一氧化氮合酶(p-eNOS)蛋白表达浓度。结果 CPB后2h,CPB组和ETA组血肌酐和尿素氮明显高于术前(P<0.05),ETA组明显低于CPB组(P<0.05);ETA组肾小管坏死评分明显低于CPB组(P<0.05);ETA组p-Akt,p-eNOS表达明显高于CPB组(P<0.05)。结论 CPB可能导致急性肾损伤,内皮素A受体拮抗剂预处理可减轻CPB后急性肾损伤。
Objective To observe the influence of endothelin A receptor antagonist pretreatment on renal function after cardiopulmonary bypass(CPB)in beagles.Methods A total of 18 male beagles were selected and allocated to 3 groups(n=6)by adopting the random number table method:sham operation group(Sham group),CPB group and endothelin A receptor antagonist(ETA)group.Sitaxsentan 0.7 mg/kg in the ETA group was infused by continuous pumping for 30 min at 1 h prior to CPB.The body temperature,mean arterial pressure(MAP)and arterial gas were collected.The concentrations of serum creatinine(Scr)and blood urea nitrogen(BUN)were detected.The renal tubular necrosis score was evaluated,and the expression levels of phosphorylated Akt(p-Akt),phosphorylated eNOS(p-eNOS)were also detected.Results Serum SCr and BUN levels at 2 h after CPB in the CPB and ETA group were significantly higher than those before operation(P<0.05),and the ETA group was obviously lower than the CPB group(P<0.05);the renal tubular necrosis score in the ETA group was obviously lower than that in the CPB group(P<0.05).Expressions of p-Akt,p-eNOS in the ETA group were significantly higher than those in the CPB group(P<0.05).Conclusion CPB might contribute to acute kidney injury,the endothelin A receptor antagonist pretreatment might alleviate acute kidney injury after CPB.
作者
白毅平
冯建国
刘力
龙翔
张亚兵
刘斌
BAI Yiping;FENG Jianguo;LIU Li;LONG Xiang;ZHANG Yabin;LIU Bin(Department of Anesthesiology,Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,China;Department of Anesthesiology,West China Hospital of Sichuan University,Chengdu,Sichuan 610000,China)
出处
《重庆医学》
CAS
2018年第10期1305-1308,1313,共5页
Chongqing medicine
基金
四川省科技厅科技支撑计划(2012FZ0121)
关键词
体外循环
肾功能
内皮素A受体拮抗剂
cardiopulmonary bypass
renal function
endothelia A receptor antagonists
