摘要
目的探讨支气管哮喘患儿外周血IL-17、IL-35水平与疾病进展的关系。方法选取2016年5月—2017年5月间住院的65例新发的支气管哮喘急性发作期患儿为研究对象,根据疾病严重程度分为间歇性支气管哮喘、轻度持续性支气管哮喘、中度持续性支气管哮喘和重度持续性支气管哮喘;同时以20例健康体检儿童为对照组。应用酶联免疫吸附试验检测所有研究对象血清IL-17及IL-35水平,并进行统计学分析。结果哮喘组IL-17为(49.9±12.5)pg/m L,高于对照组的(34.7±7.2)pg/m L,差异有统计学意义(P<0.05);IL-17水平随哮喘严重程度增加而上升,且与肺功能指标FEV1、FVC和FEV1/FVC等呈负相关(P<0.05)。哮喘组IL-35为(171.2±18.8)pg/m L,低于对照组的(264.2±33.7)pg/m L,差异有统计学意义(P<0.05);IL-35水平随哮喘严重程度增加而下降,且与肺功能指标呈正相关(P<0.05)。分别以IL-17>42.6 pg/m L和IL-35<205.8 pg/m L为截断值,均能达到较高的疾病诊断的灵敏度、特异度、准确度、阳性预测值、阴性预测值及曲线下面积(AUC);联合检测两个指标则可进一步提高诊断效果。结论哮喘儿童血清中IL-17和IL-35水平可以作为评估疾病严重程度的参考,且可能作为儿童哮喘诊断的较好的生物学标志物。
Objective To explore the relationship between the levels of IL-17 and IL-35 in peripheral blood and progression of bronchial asthma in children and the significance of their detection.Method A total of 65 hospitalized children with acute exacerbation of bronchial asthma from May 2016 to May 2017 were selected as the research object.According to the severity of the disease,those children were divided into intermittent asthma,mild persistent asthma,moderate persistent asthma and severe persistent asthma groups.At the same time,20 healthy children were taken as the control group.The serum levels of IL-17 and IL-35 were detected by enzyme-linked immunosorbent assay(ELISA),and the statistical analysis was performed.Results The IL-17 level in asthma group(49.9±12.5 pg/mL)was higher than that in control group(34.7±7.2 pg/mL),and there was significant difference(P<0.05).The level of IL-17 increased with the severity of asthma,and was negatively correlated with lung function indexes of FEV1,FVC,and FEV1/FVC(P<0.05).The IL-35 level in asthma group(171.2±18.8 pg/mL)was lower than that of control group(264.2±33.7 pg/mL),and there was significant difference(P<0.05).The level of IL-35 decreased with the increase of the severity of asthma,and was positively correlated with the index of lung function(P<0.05).When IL-17>42.6 pg/mL and IL-35<205.8 pg/mL are chosen as cut-off values respectively,both of them can achieve high diagnostic values in sensitivity,specificity,accuracy,positive predictive value,negative predictive value and area under the curve(AUC).Combination of both can further improve the diagnostic value.Conclusion The levels of L-17 and IL-35 in serum of children with asthma can be used as a reference for assessing the severity of disease,and may be a good biomarker for the diagnosis of childhood asthma.
作者
阎敏娜
项保利
张素贞
周素涛
许玉环
YAN Minna;XIANG Baoli;ZHANG Suzhen;ZHOU Sutao;XU Yuhuan(Laboratory Medicine,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei,China;Department of Respiration,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei,China)
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2018年第4期268-271,共4页
Journal of Clinical Pediatrics
