摘要
骨骼肌是维持机体功能必不可少的组织,与家养动物的产肉率等重要经济性状密切相关。近年来,高通量测序鉴定了大量与骨骼肌生成相关的长链非编码RNA(long non-coding RNA,lncRNA),它们可作为调节因子在表观调控、转录调控以及转录后调控等多个层面调控基因表达。lncRNA通过靶向关键因子参与调控骨骼肌发育的各个环节,包括骨骼肌干细胞增殖、迁移、分化,成肌细胞增殖、分化、肌管融合,肌纤维肥大和纤维类型转换等过程。本文重点归纳了lncRNA在人和小鼠骨骼肌发育中的分子调控机制,介绍了lncRNA的研究方法,综述了lncRNA在家养动物骨骼肌发育中的研究进展,分析了目前家养动物lncRNA研究所面临的困难和挑战,最后展望了未来家养动物lncRNA研究的方向,以期为进一步阐明骨骼肌生长发育的分子调控机制提供参考。
Skeletal muscle is an essential tissue to maintain the normal functions of an organism.It is also closely associated with important economic performance,such as carcass weight,of domestic animals.In recent years,studies using high-throughput sequencing techniques have identified numerous long non-coding RNAs(lncRNAs)with myogenic functions involved in regulation of gene expression at multiple levels,including epigenetic,transcriptional and post-transcriptional regulation.These lncRNAs target myogenic factors,which participate in all processes of skeletal muscle development,including proliferation,migration and differentiation of skeletal muscle stem cells,proliferation,differentiation and fusion of myocytes,muscle hypertrophy and conversion of muscle fiber types.In this review,we summarize the functional roles of lncRNAs in regulation of myogenesis in humans and mice,describe the methods for the analysis of lncRNA function,discuss the progress of lncRNA research in domestic animals,and highlight the current problems and challenges in lncRNA research on livestock production.We hope to provide a useful reference for research on lncRNA in domestic animals,thereby further identifying the molecular regulatory mechanisms in skeletal muscle growth and development.
作者
周瑞
王以鑫
龙科任
蒋岸岸
金龙
Rui Zhou;Yixin Wang;Keren Long;Anan Jiang;Long Jin(Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,China)
出处
《遗传》
CAS
CSCD
北大核心
2018年第4期292-304,共13页
Hereditas（Beijing)
基金
国家自然科学基金项目(编号:31601919,31772576,31522055),四川省教育厅项目(编号:16ZB0037),四川省省院省校科技合作研发项目(编号:2017JZ0025)和四川省青年科技创新研究团队(编号:2015TD0012)资助
关键词
长链非编码RNA
骨骼肌
作用机制
发育
家养动物
long non-coding RNAs
skeletal muscle
acting mechanism
development
domestic animals
