摘要
卵母细胞及早期胚胎在体外提取时难免受到可见光照明的刺激。但光照明对其体外发育质量的影响仍不明确。结合体外培养技术、无损成像技术(全场光学相干层析系统)及三维重构技术,在光强与曝光时间一致的条件下,首次实时原位地,从形态学角度,分析了不同可见光谱对小鼠卵母细胞及早期胚胎发育质量的影响。发现(1)与白光照明相比,蓝紫光显著降低卵母细胞生发泡破裂及第一极体排出比率,而在绿光及红光照明下,生发泡破裂及第一极体排出比率明显提高。(2)与白光组相比,蓝紫光组囊胚发生率明显下降,凋亡程度明显加大,而绿光及红光组囊胚发生率显著上调,凋亡程度明显减弱。这表明蓝紫光对小鼠卵母细胞及早期胚胎发育的损伤最大,而绿光和红光几乎无损伤。
During the process of in-vitro extraction,oocytes or early embryos can’tavoid the visible light stimulation.However,the effects of light exposure on the development quality in vitro are still unclear.This work used three technologies of in-vitro culture,noninvasive imaging(full-field optical coherence system)and three-dimensional reconstruction.Under the prerequisite of ensuring the same light intensity and the same exposure time,we were the first to usethe real-time and in-situ method to study the effects of different visible spectra on the development quality of mouse oocytes and embryos based on the morphology.It was found that(1)compared with the white light illumination,the blue-violet light significantly reduced the rates of the germinal vesicle breakdown and the first polar body extrusion of mouse oocytes.While under the greenand red light illumination,these two rates greatly increased.(2)Compared with the white light group,the blastocyst rate reduced and apoptosis increased greatly in the blue-violet light group,while the opposite was true for the green and red light groups.It indicated that blue-violet light was harmful to thedevelopment of mouse oocytes and early embryos,while the green light and redlight showed little damage.
作者
王一鸣
张冰
王婷
张纾
于吉
吴闯
田宁
WANG Yi-ming;ZHANG Bing;WANG Ting;ZHANG Shu;YU Ji;WU Chuang;TIAN Ning(College of Physics Science and Technology,Shenyang Normal University,Shenyang 110000,China;College of Science,Huazhong Agricultural University,Wuhan 430000,China;Experimental Teaching Center,Shenyang Normal University,Shenyang 110000,China)
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2018年第3期918-922,共5页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(11604112)
辽宁省自然科学基金项目(2015020715)
沈阳师范大学优秀人才支持计划(BS201433)
沈阳师范大学自主科研计划(L201514)
沈阳师范大学博士启动计划(51600209)资助
关键词
可见光照明
卵母细胞
早期胚胎
全场光学相干层析技术
体外培养
Visible light illumination
Oocyte
Early embryo
Full-field optical coherence tomography
In-vitro culture
