摘要
目的:研究头状四照花的抗疟活性成分,为寻找新型抗疟药物奠定物质基础。方法:利用β-羟高铁血红素形成抑制实验进行体外抗疟活性筛选,采用柱色谱和波谱技术进行活性成分追踪分离和结构鉴定。结果:从头状四照花枝叶中得到4个化合物,分别鉴定为:儿茶素(1)、槲皮素(2)、异槲皮苷(3)和2α-羟基乌索酸(4);其中黄酮类化合物1~3具有不同程度的β-羟高铁血红素形成抑制活性,化合物2的IC50值为(155.7±6.5)μg/m L,化合物1和3在最高浓度时,抑制率分别为32.2%和28.4%。结论:头状四照花提取物具有一定的β-羟高铁血红素形成抑制活性,其活性物质基础可能为黄酮类化合物。
Objective:To study the antimalarial activity of Dendrobenthamia capitata(Si-Zhao-Hua),and to lay the foundation for the development of novel antimalarial drugs from plants.Methods:Aβ-hematin formation inhibition experiment was used to evaluate antimalarial activity in vitro.The compounds were isolated and purified by column chromatography,and their structures were elucidated based on the analyses of spectral data and physicochemical properties.Results:Four compounds were obtained from twigs and leaves of D.capitata and identified as catechin(1),quercetin(2),isoquercitrin(3),and 2α-hydroxyursolic acid(4).All compounds were tested for the antimalarial activity byβ-hematin formation inhibition assay,compound 2 showed the IC50 value of(155.7±6.5)μg/mL,and the inhibition ratios of compounds 1 and 3 were 32.2%and 28.4%at the highest concentration,respectively.Conclusion:The extract of D.capitata have certainβ-hematin formation inhibition activity,the base of its active substance may be flavonoid.
作者
张盼盼
肖朝江
刘健
邱琳
沈怡
董相
姜北
Zhang Panpan;Xiao Chaojiang;Liu Jian;Qiu Lin;Shen Yi;Dong Xiang;Jiang Bei(Institute of Materia Medica of Dali University,Dali,Yunnan 671000,China)
出处
《大理大学学报》
CAS
2018年第2期1-5,共5页
Journal of Dali University
基金
国家自然科学基金资助项目(81460532)
关键词
头状四照花
抗疟活性
β-羟高铁血红素
化学成分
Dendrobenthamia capitata
antimalarial activity
β-hematin
chemical constituents
