摘要
目的为解决整形外科骨缺损修复,促进移植骨成活,应用干细胞联合培养体系复合部分脱蛋白生物骨(PDPBB),对机体胫骨缺损进行修复.方法新西兰大耳兔18只,分别抽取骨髓液及外周血提取骨髓间充质干细胞(BMSCs)及外周血内皮祖细胞(EPCs),构建联合培养体系后,复合PDPBB构建组织工程骨;于兔胫骨制造1 cm长骨缺损,将组织工程骨植入缺损区,分别于术后14 d、28 d及2个月观察骨缺损修复的情况.结果 BMSCs组、联合培养细胞组和空白组各个时间点及组间的吸光度值比较,差异均有统计学意义(P<0.001);组织工程骨植入体内后骨胶原蛋白含量逐渐升高,各分组差异有统计学意义(P<0.01);复合联合干细胞体系的PDPBB修复骨缺损能力最强,修复了胫骨缺损区的结构和功能.结论 EPCs与BMSCs联合干细胞体系复合PDPBB构建的组织工程骨是良好的骨缺损修复材料.
Objective To reconstruct the deformity of appearance and function of patients with bone defect,co-cultured system with two stem cells were combined with partial deproteinized biological bone to reconstruct the defect of tibia which is one of the main weight-bearing bone.Methods The bone marrow and peripheral blood were harvested form 18 New Zealand rabbits to isolated bone marrow stem cells and epithelial progenitor cells,and engineering bone was constructed with co-cultured system with these two stem cells and partial deproteinized biological bones;about 1 CMof bone defect of each rabbit was made with bone rongeur,then engineering bones were transplanted into the defect area,the osteogenesis and bone defect recovery were observed on day 14,28 and month 2.Results The difference of absorbance values of BMSCs group,co-cultured cell group and blank group at each time point and between groups were all statistically significant(<0.001),and the collagen content of bone tissue increased gradually after implantation of tissue engineered bone,and the difference between each group was statistically significant(<0.001).The repairment of bone defect with the PDPBB combined withBMSCs and EPCs system has the strongest ability to repair the structure and function of the tibial defect area.Conclusion The engineering bone constructed with two stem cells and partial deproteinized bone is a good material for bone defect reconstruction.
作者
赵娴
韩雪松
周箐竹
李杉
刘流
王松梅
ZHAO Xian;HAN Xue-song;ZHOU Qing-zhu;LI Shan;LIU Liu;WANG Song-mei(Dept.of Plastic Surgery,The First Affiliated Hospital of Kumming Medical University,Kunming Yunnan 650031;The Public Health College of Kumming Medical University,Kunming Yunnan 650500;The Life Science College of Yunnan University,Kunming Yunnan 650031,China)
出处
《昆明医科大学学报》
CAS
2018年第3期11-18,共8页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81460296)
云南省应用基础研究面上基金资助项目(2017FB121)
云南省卫生科技后备人才计划资助项目(H-201631)
关键词
组织工程骨
内皮组细胞
骨髓间充质干细胞
联合培养
骨缺损修复
Engineering bone
Epithelial progenitor cell
Bone marrow mesenchymal stem cell
Co-culture
Bone defect repairment
