摘要
目的观察以益肾填精法为主的茸菖胶囊对无镁诱导的癫痫细胞模型海马神经元形态及存活状态的影响,探讨其对损伤神经元的保护作用及机制。方法原代培养新生SD大鼠海马神经元,建立无镁诱导的癫痫细胞模型,随机分为正常组(正常细胞外液)、模型组(无镁细胞外液)、对照组(无镁+3-甲基腺嘌呤)和5%、10%、20%茸菖胶囊组(无镁+不同浓度茸菖胶囊),采用倒置相差显微镜观察各组神经元形态学变化及凋亡情况,检测海马神经元活性及海马神经元损伤程度。结果 (1)形态学变化:各时间点不同浓度茸菖胶囊组细胞较模型组海马神经元形态损伤减轻,胞体较完整,突起较清楚,存活细胞量多;(2)海马神经元活性:与正常组比较,模型组海马神经元细胞活力显著降低,差异有统计学意义(P<0.05);与模型组比较,5%、10%、20%茸菖胶囊组显著提高无镁诱导的海马神经元的细胞活力,差异有统计学意义(P<0.05);(3)海马神经元损伤程度:与模型组比较,5%、10%、20%茸菖胶囊组可显著减少无镁诱导所致的海马神经元内乳酸脱氢酶释放量,差异有统计学意义(P<0.05),并具有浓度依赖性;与正常组比较,模型组海马神经元乳酸脱氢酶释放量显著升高,差异有统计学意义(P<0.05);5%、10%、20%茸菖胶囊组海马神经元内乳酸脱氢酶释放量随其浓度升高而逐渐降低。结论 (1)茸菖胶囊能降低无镁诱导的癫痫细胞模型海马神经元乳酸脱氢酶释放量,维持细胞膜的完整性及线粒体功能,提高细胞活力并降低海马神经元凋亡率,起到保护海马神经元的作用。(2)茸菖胶囊提高对无镁诱导癫痫细胞模型神经元的活性,抑制海马神经元的凋亡,其神经保护作用可能与调控神经元自噬有关。
Objective To observe the effect of Rongchang capsule characterized by tonifying kidney and replenishing essence on the morphology and survival status of magnesium free epileptic models of hippocampal neuron,and discuss its protection effect on injured neuron and the probable mechanism.Methods Establish the magnesium free epilepsy cell model by culturing the hippocampal neuron of newborn SD rats,which were randomly divided into normal group(normal extracellular fluid),model group(magnesium free extracellular fluid),control group(magnesium free+3 MA),and the Rongchang capsule group of 5%,10%and 20%concentration(magnesium free+Rongchang capsule of different concentration).The morphological changes and apoptosis of neutron cell of each group were observed through inverted phase contrast microscope;the activities of neutron cell were identified,and the injury degrees of neuron cell were determined.Results(1)Morphological changes:compared to the model group,the morphological damage of Chinese medicine cerebrospinal fluid group of different concentration were alleviated at each time point;the cell bodies were comparatively complete,the outgrowth was clear and there were more surviving cells.(2)Hippocampal neuron activity:compared with normal group,the activity of hippocampal neuron in model group was reduced remarkably(P<0.05);the activity of magnesium free hippocampal neuron was greatly increased in the group processed with different concentration of Rongchang capsule compared with model group(P<0.05).(3)Hippocampus neuron damage degree:compared with model group,Rongchang capsule(low,medium,and high dose)group could significantly reduce the LDH release volume in magnesium free hippocampal neuron,being concentration dependent(P<0.05);compared with normal group,the LDH release volume in hippocampal neuron of model group was significantly higher(P<0.05);the LDH release volume in hippocampal neuronof Rongchang capsule(low,medium,and high dose)group decreased with the increase of concentration.Conclusion(1)Rongchang capsul
作者
戎萍
赵伟
张喜莲
王伟
张雪荣
马融
RONG Ping;ZHAO Wei;ZHANG Xilian;WANG Wei;ZHANG Xuerong;MA Rong(Department of Pediatrics,the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China)
出处
《中国中西医结合儿科学》
2018年第1期1-5,共5页
Chinese Pediatrics of Integrated Traditional and Western Medicine
基金
国家自然科学基金项目(81373691)
关键词
癫痫
无镁诱导
海马神经元
凋亡
自噬
茸菖胶囊
大鼠
Magnesium free induction
Epilepsy
Hippocampal neurons
Apoptosis
Autophagy
Rongchang capsule
Rat
