摘要
目的:分析下咽鳞状细胞癌患者外周血T淋巴细胞亚群和NK细胞活性情况,旨在为相关研究工作提供参考资料。方法:择取2011年6月-2016年5月本院收治的95例下咽鳞状细胞癌病患为病例组,另取此期间内来本院接受健康检查的72例志愿者为正常组。使用流式细胞仪对病患开展相关检查,分析病例组与正常组的淋巴细胞亚群、NK活性情况以及不同临床分期的下咽癌病患外周血T淋巴细胞亚群以及NK细胞活性之间的关系。结果:与正常组相比,病例组的NK细胞、总B淋巴细胞、总T淋巴细胞、辅助/抑制T细胞、辅助T细胞偏低(P<0.05),活化T细胞以及抑制T细胞偏高(P<0.05)。Ⅰ~Ⅱ期病患外周血总T淋巴细胞比Ⅲ~Ⅳ期者稍低(P>0.05)。Ⅰ~Ⅱ期病患辅助T淋巴细胞比Ⅲ~Ⅳ期者略高(P>0.05)。Ⅰ~Ⅱ期病患抑制T淋巴细胞值比Ⅲ~Ⅳ期者低(P<0.01)。Ⅰ~Ⅱ期病患辅助/抑制T淋巴细胞值比Ⅲ~Ⅳ期者高(P<0.01)。Ⅰ~Ⅱ期病患外周血总B淋巴细胞值比Ⅲ~Ⅳ期者高(P<0.05)。Ⅰ~Ⅱ期病患的活化T细胞和Ⅲ~Ⅳ期者相近(P>0.05)。Ⅰ~Ⅱ期病患外周血NK细胞数比Ⅲ~Ⅳ期者略高(P>0.05)。结论:下咽鳞状细胞癌病患的免疫功能异常,且随着疾病的进展,细胞免疫和体液免疫均有所降低,证实免疫功能紊乱在疾病进展中发挥了一定作用。经流式细胞设备对外周静脉血淋巴细胞亚群以及NK细胞活性情况检测,可实现动态化检测癌症病患某阶段细胞免疫功能变化详情,此法方便简单,在指导治疗方案制定以及监测疾病预后方面,均体现出了一定效果,值得进一步推广。
Objective:To analyze the peripheral blood T lymphocyte subsets and NK cell activity in patients with hypopharyngeal squamous cell carcinoma,and to provide reference for related research work.Method:From June 2011 to May 2016 in our hospital 95 cases of hypopharyngeal squamous cell cancer were selected the case group,the other from this period came to our hospital for health examination of 72 volunteers were selected as the normal group.Using the relevant examination by flow cytometry,the relationship between the case group and the normal group of lymphocyte subsets and the activity of NK and different clinical staging of hypopharyngeal cancer patients peripheral blood T lymphocyte subsets and NK cell activity were analyzed.Result:Compared with the normal group,NK cells,total B lymphocyte,total T lymphocytes,helper/suppressor T cells,and helper T cells were lower in the case group(P<0.05).The activation of T cells and inhibition of T cells were higher(P<0.05).Peripheral blood total T lymphocyte ofⅠ-Ⅱstage was similarity toⅢ-Ⅳstage(P>0.05).Helper T lymphocytes ofⅠ-Ⅱstage was slightly higher than that ofⅢ-Ⅳstage(P>0.05).The value of T lymphocyte suppression ofⅠ-Ⅱstage was lower than that ofⅢ-Ⅳstage(P<0.01).T lymphocyte count ofⅠ-Ⅱstage was higher than that ofⅢ-Ⅳstage(P<0.01).The total B lymphocyte count in peripheral blood ofⅠ-Ⅱstage was higher than that ofⅢ-Ⅳstage(P<0.05).The activated T cells ofⅠ-Ⅱstage was similar toⅢ-Ⅳstage(P>0.05).The number of NK cells in peripheral blood of patients ofⅠ-Ⅱstage was slightly higher than that ofⅢ-Ⅳstage(P>0.05).Conclusion:The immune function of hypopharyngeal squamous cell cancer is abnormal,and with the progress of the disease,cellular immunity and humoral immunity are decreased,confirmed that the disorder of immune function play a certain role in the progression of the disease.The flow cytometry equipment of peripheral blood lymphocyte subsets and NK cell activity are detected,can realize the dynamic detection of cancer
作者
姜元芹
陈爱民
黄煦格
骆献阳
张炳煌
蔡成福
周燚
刘存山
JIANG Yuanqin;CHEN Aimin;HUANG Xuge(The First Affiliated Hospital of Xiamen University,Xiamen 361003,China)
出处
《中国医学创新》
CAS
2017年第35期63-66,共4页
Medical Innovation of China
关键词
下咽鳞状细胞癌
外周血
T淋巴细胞亚群
NK细胞
Hypopharyngeal squamous cell carcinoma
Peripheral blood
T lymphocyte subsets
NK cells
