摘要
目的探讨抗酶抑制剂(AZIN)对前列腺癌细胞PC3鸟氨酸脱羧酶(ODC)表达水平及增殖的影响。方法将siRNA-AZIN转染前列腺癌PC3细胞,分别用RT-PCR及Western blot检测抗酶(AZ)、AZIN及ODC在mRNA和蛋白水平的表达,MTT检测细胞增殖。结果 siRNA-AZIN转染前列腺癌PC3细胞后,AZIN RNA表达水平降低(P<0.01);PC3细胞AZIN蛋白表达水平降低(P<0.05),且使ODC的表达水平降低(P<0.05);PC3细胞增殖能力明显减弱(P<0.05)。结论 siRNA-AZIN可下调前列腺癌细胞PC3中ODC的表达。
Objective To evaluate whether down-regulating antizyme inhibitor(AZIN)can regulate the expression of ornithine decarboxylase(ODC)and the proliferation of prostate cancer cell PC3 or not.Methods siRNA-AZIN transfected prostate cancer cell PC3,the level of antizyme(AZ),AZIN and ODC were measured by RT-PCR and westernblot.MTT was used to measure the proliferation of cells.Results The mRNA level of AZIN declined(P<0.01);the protein level of AZIN and ODC declined(P<0.05).Knockdown of AZIN significantly inhibited the proliferation of PC3(P<0.05).Conclusions Transfecting siRNA-AZIN can decrease the level of AZIN,then the decline level of ODC inhibits the proliferation of PC3.
作者
金涛
李倩
欧洁
王艳林
吴红艳
JIN Tao;LI Qian;OU Jie;WANG Yanlin;WU Hongyan(People s Hospital of Three Gorges University,the First People s Hospital of Yichang,Yichang 443002,China;Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy,Medical College of Three Gorges University,Yichang 443002,China;.Dept.of Immunology,Medical College of Three Gorges University,Yichang 443002,China)
出处
《基础医学与临床》
CSCD
2018年第4期475-479,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(81402557)
