摘要
文中探讨了喷施富硒营养液对蓝莓营养品质和抗氧化功能的影响。在蓝莓座果后期、膨大期叶面喷施有机硒营养液,通过分光光度法、凯氏定氮法、原子荧光光度计法及量子共振等方法测定蓝莓中主要营养成分、功能性元素及SOD活性,分析蓝莓富硒后的营养品质;用活性氧自由基荧光探针DCFH-DA(2,7-dichlorodihydrofluorescein-diacetate)检测富硒后的蓝莓对H_2O_2诱发的细胞自由基清除率以及对小鼠抗氧化酶活性的影响。结果表明:施有机硒蓝莓浆果中蛋白质、可溶性糖、可溶性固形物、VC、酚、黄酮、硒的含量及SOD的活性显著高于施清水及无机硒组蓝莓,VE、花色素的含量也有一定程度的提高;清水组和无机硒组蓝莓的自由基清除率分别为10.34%和19.94%,有机硒组则高达27.49%,与对照组相比较,饲有机硒蓝莓小鼠的肝脏中丙二醛(malondialdehyde,MDA)含量降低了51.53%,血清中超氧化物歧化酶(superoxide dismutase,SOD)及谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性分别升高了45.93%及55.94%,无机硒蓝莓小鼠的肝组织中MDA含量降低了29.81%,血清中SOD和GSH-Px的活性分别升高了29.62%和38.02%,有机硒蓝莓对小鼠抗氧化酶的影响大于无机硒蓝莓。施有机硒营养液优化了蓝莓营养谱,提高了蓝莓对机体的抗氧化能力,且对机体肝组织氧化损伤有显著的保健功效。
The effect of selenium nutrient solution were studied on nutrition quality and antioxidant function of blueberry,so as to provide evidence in developing new natural function food.Solution of selenium nutrient was sprayed on the leaves of blueberry at the late stage of fruit setting and the fruit formation.The fruit nutrition qualities of blueberry,such as nutrient composition,function elements and superoxide dismutase(SOD)activity were analyzed by spectrophotometric method,kjeldah method,atomic fluorescence photometer method and QRS method.In order to study the effects of organic selenium blueberry on ROS in cells,the HepG2 cell were pretreated with organic selenium blueberry before exposure to DCFH-DA,and then the variation of ROS was detected using laser scanning confocal microscope.For the antioxidant level experiment of organic selenium blueberry in vivo,the mouse was fed with organic selenium blueberry for one month.Then the blood and liver of mice were collected for analysis.The results showed that compared with the water and sodium selenite groups,the content of protein,soluble sugar,vitamin C,total phenolics,flavone,selenium,and the activity of SOD raised significantly treated with selenium solution.The content of vitamin E and anthocyanidin sprayed selenium nutrient solution was also improved in a certain degree.The increase of ROS was obviously inhibited by organic selenium blueberry.Comparing to control group,When the mouse were feeded with organic selenium blueberry or selenium blueberry,the content of MDA was obviously reduced to 51.53%or 29.81%,the activity of SOD was obviously increased to 45.93%and 29.62%,and the activity GSH-Px was increased to 55.94%or 38.02%.The influence of antioxidant enzymes by organic selenium blueberry was greater.Our results indicated that organic selenium blueberry has a significant effect against oxidative damage by nutrition elements optimization and immune function enhancement.
作者
李春霞
刘伟
陈凌云
姜文仪
蒋光月
黄蓓
LI Chun-xia;LIU Wei;CHEN Ling-yun;JIANG Wen-yi;JIANG Guang-yue;HUANG Bei(School of Life Sciences,Anhui University,Hefei 230601,China;Agricultural Science Academy of Anhui Province,Hefei 230031,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2018年第3期179-185,共7页
Food and Fermentation Industries
基金
安徽省科技攻关项目(1301032140
1501041177)
关键词
蓝莓
有机硒
营养品质
抗氧化能力
blueberry
organic selenium
nutrition quality
antioxidant capacity
