摘要
目的研究蝮蛇粉不同配伍方对老龄小鼠体内抗氧化酶活性的影响。方法将10月龄ICR雄性小鼠按4%溶血液中丙二醛(MDA)水平随机分为蝮蛇粉(78mg/kg)组、蝮蛇粉+姜黄提取物(52+15.6mg/kg)组、蝮蛇粉+姜黄提取物+茶多酚(52+15.6+65mg/kg)组、阴性对照组,每组10只。各组灌胃给予相应药物,阴性对照组给予相应溶剂,每天1次,连续给药30天。采用试剂盒法检测4%溶血液中MDA水平、全血中谷胱甘肽(GSH)含量、血清中总抗氧化能力(T-AOC)和蛋白质羰基含量以及超氧化物歧化酶(SOD)的活力变化。结果与阴性对照组比较,其余各组小鼠4%溶血液中MDA水平显著降低,全血中GSH含量增加,血清蛋白质羰基含量明显降低、T-AOC含量和SOD活力显著提升(P<0.05,P<0.01);与蝮蛇粉组比较,蝮蛇粉+姜黄提取物组小鼠4%溶血液中MDA水平显著降低,全血中GSH含量增加,血清蛋白质羰基含量明显降低、T-AOC含量和SOD活力显著提升(P<0.05,P<0.01);与蝮蛇粉+姜黄提取物+茶多酚组比较,蝮蛇粉+姜黄提取物组小鼠各指标变化无明显差异。结论蝮蛇粉与姜黄提取物合用可有效改善老龄小鼠体内抗氧化能力,可起到延缓衰老作用。
Objective To study the effects of different compatibilities of viper powder on antioxidant activity in aged mice. Methods According to the level of MDA in 4%hemolysis solution,a total of 10 month old ICR male mice were randomly divided into four groups:viper powder group,viper powder+turmeric extract group,viper powder+turmeric extract+tea polyphenols group,and negative control group,with 10 mice in each group.Each group was given the corresponding medicine,and the negative control group was given the corresponding solvent,1 times a day,continuous administration 30 d.The contents of malondialdehyde(MDA),total glutathione(GSH)in whole blood,total antioxidant capacity(T-AOC)in serum and protein oxidation product protein carbonyl content and the activity of superoxide dismutase(SOD)in 4%hemolytic fluid were detected by kit method. Results Compared with the negative control group,the level of MDA in 4%hemolysis was significantly decreased,GSH content in whole blood increased,the carbonyl content of serum protein decreased significantly,the content of T-AOC and SOD activity was significantly increased in other each groups(P<0.05 or P<0.01);compared with viper powder group,the level of MDA in 4%hemolysis solution of viper powder+turmeric extract group was significantly decreased,GSH content in whole blood was increased,the carbonyl content of serum protein was significantly decreased,T-AOC content and SOD activity were significantly increased(P<0.05 or P<0.01).Compared with viper powder+turmeric extract+tea polyphenol group,there was no significant difference in each index between viper powder+turmeric extract group. Conclusion The combined use of viper powder and turmeric extract can effectively improve the activity of antioxidant in aged mice to reach the effect of delaying senescence.
作者
常明
叶勇
阳洁
辛丽娜
王羽中
王捷
卢奕
CHANG Mimg;YE Yong;YANG Jie;XIN Li-na;WANG Yu-zhong;Wang Jie;LU Yi(Guangxi Medical University,Nanning,Guangxi,530021,China;Guangxi-ASEAN Food and drug safety inspection and Testing Center,Nanning,Guangxi,530001,China;Phamaceutical Factory of Guangxi Medical University,Nanning,Guangxi,530021,China)
出处
《蛇志》
2018年第1期1-3,26,共4页
Journal of Snake
基金
南宁市科学研究与技术开发计划项目重点研发计划(编号:20173158-3)
南宁市青秀区科学研究与技术开发计划项目重大专项(编号:2015Z01)
