摘要
目的:探讨乳腺癌TAMs/MCF-7细胞共培养体系中血管内皮细胞生长因子(VEGF)过表达对两种细胞中B淋巴细胞瘤-2基因(Bcl-2)表达的影响。方法:应用佛波酯(PMA)及白细胞介素-4(IL-4)体外诱导TAMs细胞,Transwell非接触式共培养TAMs和MCF-7细胞,四甲基偶氮唑蓝(MTT)法检测共培养后MCF-7细胞增殖情况;免疫蛋白印记法(Westernblot)检测TAMs/MCF-7细胞共培养体系中过表达VEGF对两种细胞Bcl-2表达的影响。结果:应用PMA及IL-4体外诱导THP-1成为TAMs细胞,MCF-7与TAMs细胞共培养24、48 h后,细胞增殖活性较对照组分别上升了16.16%和33.99%。TAMs、MCF-7细胞分别加入VEGF及共培养体系上清,两种细胞Bcl-2表达均明显高于对照组,差异有统计学意义(P<0.05)。结论:肿瘤通过分泌VEGF等趋化因子,招募并活化TAMs。通过肿瘤微环境内VEGF/Bcl-2旁分泌环路作用,影响肿瘤的增殖凋亡及进展。
Objective:To explore the effect of VEGF overexpression on the Bcl-2 of TAMs/MCF-7 cell co-culture system.Method:Application of PMA and IL-4 cells induced THP-1 cell differentiation into TAMs in vitro;TAMs and MCF-7 cell were co-cultured in non-contact Transwell system.MCF-7 cells’proliferation status after co-culture were detected by MTT method;Effect of VEGF over-expression in co-cultured system on Bcl-2 level s in the two cell lines by Western blot assay.Results:PMA and IL-4 induced THP-1 cell become TAMs in vitro.After co-cultured with TAMs 24h,48h,MCF-7 cell’s proliferation activity increased by 16.16%and 33.99%vs the control group respectively.TAMs,MCF-7 cells were added VEGF and the supernatant of co-culture system respectively,then Bcl-2 levels in both cells were significantly higher,the difference was statistically significant(p<0.05).Conclusion:Tumors secrete VEGF and other chemokines to recruit and activate TAMs.Proliferation,apoptosis and progression of tumor was affected by VEGF/Bcl-2 paracrine loop in tumor microenvironment.
作者
刘伟
安杰
侯会池
李康
李志
甄四虎
王少文
LIU Wei;AN Jie;HOU Huichi;LI Kang;LI Zhi;ZHEN Sihu;WANG Shaowen(Department of General Surgery,The Bethune International Peace Hospital,Shijiazhuang 050082,China;Department of Pathology,The Bethune International Peace Hospital,Shijiazhuang 050082,China)
出处
《中国现代普通外科进展》
CAS
2017年第12期925-928,共4页
Chinese Journal of Current Advances in General Surgery
基金
河北省卫生计生委基金资助项目(20160004)
