摘要
目的探讨血流感染患者血液中分离出的鲍曼不动杆菌的耐药机制及基因同源性,为多重耐药的鲍曼不动杆菌感染提供防治依据。方法收集该院2015年1月至2016年6月共14例由鲍曼不动杆菌引起血流感染的住院患者的不同部位标本,共分离出38株鲍曼不动杆菌。所有菌株按照统一的操作规程进行药敏试验,聚合酶链式反应(PCR)扩增及琼脂糖凝胶电泳分析碳青霉烯酶基因,并采用多位点序列分型(MLST)及eBURST分析软件进行同源性研究。结果38株鲍曼不动杆菌均为多重耐药株,均检测到OXA-23-like、OXA-51-like基因及ISAbal序列,OXA-23-like基因与ISAbal序列相关;MLST分型显示38株鲍曼不动杆菌主要由ST195型(55.3%)、STn-2型(15.8%)及STn-3型(10.5%)构成。eBURST分析提示主要流行克隆为CC92,占86.8%。结论多重耐药的鲍曼不动杆菌携带相同耐药基因,且菌株存在较高的遗传同源性,提示存在克隆传播,为降低医院内鲍曼不动杆菌相关血流感染风险提供了分子生物学依据。
Objective To investigate the drug resistant mechanism and the genetic homology of Acinetobacter baumannii(A.ba)isolated from blood in the patients with bloodstream infection so as to provide a basis for the prevention and treatment of multi-drug resistant A.ba infection.Methods The different sites of samples from the inpatients with bloodstream infection caused by A.ba were collected from January 2015 to June 2016.A total of 38 strains of A.Ba were isolated.The drug susceptibility test of all bacterial strains was performed according to the uniform operating specification.The carbapenemase gene was amplified by PCR and its products were analyzed by agarose gel electrophoresis.The homology study was performed by using multilocus sequence typing(MLST)and eBURST analysis software.Results All 38 strains of A.ba were multi-drug-resistant.And in all of them,OXA-23-like and OXA-51-like gene,and ISAbal sequence were detected.OXA-23-like gene was associated with ISAbal sequence;the MLST typing showed that 38 A.ba strains mainly included the type ST195(55.3%),STn-2(15.8%)and STn-3(10.5%).The eBURST analysis indicated that the main prevalent clones were CC92,accounting for 86.8%.Conclusion Multi-drug resistant A.ba carries the same drug-resistant gene and its strain has higher genetic homology,indicating that the clone spread exists,which provides a molecular biological basis for reducing the risks of A.ba related bloodstream infection in hospital.
作者
罗媛
张任飞
侯钧
LUO Yuan;ZHANG Renfei;HOU Jun(Department of Clinical Laboratory,Mianyang Municipal Third People′s Hospital,Mianyang,Sichuan 621000,China)
出处
《国际检验医学杂志》
CAS
2018年第3期303-307,共5页
International Journal of Laboratory Medicine
关键词
鲍曼不动杆菌
多重耐药菌
血流感染
耐药基因
同源性
Acinetobacter baumannii
multi-drug resistant
bloodstream infection
drug-resistant gene
homology
