摘要
产香微生物的研究对天然香料的绿色生产具有深远的意义。本文从醇化中期的烟叶上分离得到一株细菌HD-7,根据形态学、生理生化以及16S rRNA基因序列等特征,对产香菌株HD-7进行了初步鉴定,并利用气相色谱-质谱(GC-MS)联用技术分析其发酵液的成分。结果表明,菌株HD-7初步被鉴定为短小芽孢杆菌(Bacillus pumilus);其GC-MS检测出的物质中含有较多的香气物质,主要的增香成分有丙酸乙酯,乙酸,乙酸丙酯及3-羟基-2-丁酮(乙偶姻)等,其含量分别占25.56%,12.68%,6.80%,4.86%。其中丙酸乙酯及3-羟基-2-丁酮等都是广泛使用的香料。产香细菌HD-7在生产香料上具有很大的研究价值。
Aroma-producing microorganisms have considerable promise for producing natural flavors and fragrances in an environment-friendly way.In this study,morphological characterization,physiological and biochemical test,and 16S rDNA sequence analysis were performed to identify aroma-producing strain HD-7.Additionally,the aroma compositions from aroma-producing strain HD-7 fermentation were analyzed by gas chromatography-mass spectrometry(GC-MS).The result showed that the strain HD-7 was a Bacillus pumilus.A large number of aroma compositions were detected by GC-MS,and the main components included ethyl propionate,acetic acid,n-Propyl acetate,3-hydroxy-2-Butanone with the contents of 25.56%,12.68%,6.80%,4.86%,respectively,among which the ethyl propionate and 3-hydroxy-2-Butanone were widely used as flavors and fragrances.The aroma-producing strain HD-7 is of great application value for production of natural flavors and fragrances.
作者
吴丽君
梁开朝
王帆
高锐
王毅
段如敏
刘好宝
张海波
白晓莉
WU Li-jun;LIANG Kai-chao;WANG Fan;GAO Rui;WANG Yi;DUAN Ru-min;LIU Hao-bao;ZHANG Hai-bo;BAI Xiao-li(R&D center of China tobacco Yunnan industrial Co.,Ltd,Kunming 650202,China;Hainan Cigar research Institute Hainan Provincial Branch of China National Tobacco Corporation,571100,China;CAS Key Laboratory of Biobased Materials,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第2期246-250,共5页
Natural Product Research and Development
基金
云南省科技厅项目(2015IA026)
