摘要
为探索鱼肉品种快速鉴别方法,以鱼类小清蛋白基因为靶标,设计了多宝鱼、银鲳鱼、金枪鱼、暗纹东方鲀、青石斑鱼、带鱼、大黄鱼以及东星斑鱼8种鱼的通用引物以及特异性探针,利用可视芯片技术,建立了一种鱼肉品种的高通量快速检测方法。该方法特异性好、灵敏、通量高,可同时准确鉴别8种鱼成分,检测灵敏度均可达0.1 ng/μL,且结果肉眼可见。该方法在鱼肉及其制品掺假现场快速筛查方面具有较大应用潜力。
A method for the rapid and high-throughput identification of fish species using thin-film biosensor chips was developed.Based on the parvalbumin gene,a set of universal primers and specific probes were designed for eight fish species including Turbot,Silvery pomfret,Tuna,Obscura puffer,Indigo hamlet,Hairtail,Large yellow croaker and Miniatus grouper.The assay,with the characteristic of high specificity and throughput,could produce signals visualized by the unaided eye without any specific instruments.And the detection limit reached to 0.1 ng/μL.This method provided an important technical means for identifying eight fish species,which could be utilized in rapid screening for fish adulteration.
作者
韩建勋
陈颖
王斌
张九凯
马秀丽
HAN Jian-xun;CHEN Ying;WANG Bin;ZHANG Jiu-kai;MA Xiu-li(.Agro-product Safety Research Center,Chinese Academy of Inspection and Quarantine,Beijing 100176,China;College of Food Science&Nutritional Engineering,China Agriculture University,Beijing 100083,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2018年第2期174-179,共6页
Journal of Instrumental Analysis
基金
国家重点研发计划资助(2016YFD0401104)
关键词
可视芯片
鱼
鉴别
高通量
thin-film biosensor chip
fish
identification
high-throughput
