摘要
目的观察腺病毒载体Ad-pre-microRNA-494抑制Survivin表达对人膀胱尿路上皮癌UM-UC-3细胞周期及凋亡的影响。方法使用已构建的腺病毒载体Ad-pre-microRNA-494感染人膀胱尿路上皮癌UM-UC-3细胞,采用RT-PCR法、Western blot法、流式细胞计数法检测感染前后Survivin基因及蛋白的表达差异和细胞生长周期及凋亡情况。结果实验组细胞与空病毒载体组细胞相比较,Survivin基因表达下调,细胞凋亡率显著增加(P<0.01)。结论 Ad-premicroRNA-494能有效抑制UM-UC-3细胞中Survivin基因表达,从而诱导UM-UC-3细胞凋亡,这为下一步的动物实验研究奠定了基础。
Objective To investigate the effect of adenovirus vector Ad-pre-microRNA-494 on the inhibition of Survivin gene expression and the apoptosis induction of UM-UC-3 cells. Methods Adenovirus vector Ad-pre-microRNA-494 was constructed to infect human bladder transitional cell carcinoma UM-UC-3 cells.RT-PCR,Western blot and flow cytometry assay were used to detect the expression of Survivin gene,cell growth cycle and apoptosis and compare the difference between before and after infection. Results The expression of Survivin gene in the experimental group downregulated compared with empty vector group,and the apoptosis rate was significantly increased(P<0.01). Conclusions Ad-pre-microRNA-494 can effectively inhibit the expression of Survivin gene in UM-UC-3 cells and induce the apoptosis of UM-UC-3 cells,which lays the foundation for the further animal experiment.
作者
王礼平
卢晓明
阳东荣
李兰
王进峰
周国洋
尹九湖
刘亚东
董彬彬
WANG Li-ping;LU Xiao-ming;YANG Dong-rong;LI Lan;WANG Jin-feng;ZHOU Guo-yang;YIN Jiu-hu;LIU Ya-dong;DONG Bin-bin(Department of Urology,Yancheng Third People's Hospital,Yancheng 224000,China)
出处
《现代泌尿生殖肿瘤杂志》
2018年第1期43-47,共5页
Journal of Contemporary Urologic and Reproductive Oncology
基金
盐城市医学科技发展计划项目(YK2017110)
