摘要
目的通过高通量16S r DNA测序,探讨不同中医证型原发性失眠患者肠道菌群差异。方法选择原发性失眠患者65例,按中医证型分为肝郁化火组22例、心脾两虚组17例、阴虚火旺组26例,健康体检者47例为对照组。采用高通量16S r DNA测序方法分析患者便菌群结构,QIIME软件与R语言stats包等分析菌群差异。结果共得到1226个不同的基因序列分类单元(OTUs),4组间差异有统计学意义的OTUs共180个(P<0.05),表明样品有丰富的菌落。肝郁化火组、阴虚火旺组测序序列的定位个数多于心脾两虚组及对照组(P<0.05)。非加权Uni Frac分析显示,组间差异显著大于组内差异,表明分组有意义(R=0.103,P=0.002),提示肠道菌群多样性与失眠不同证型高度相关。属水平在不同组间明显差异共57个属(P<0.05),所有物种水平不同组间明显差异共115个物种(P<0.05)。对照组优势菌属包括:普雷沃氏菌属(prevotella)、巨单胞菌属(megamonas)、梭菌属(clostridiumⅪ、clostridiuumⅩⅧ)、魏斯氏菌属(weissella)、拟普雷沃氏菌属(alloprevotella);肝郁化火组优势菌属包括:考拉杆菌属(phascolarctobacterium)、黄杆菌属(flavonifractor)、埃格特菌属(eggerthella)、嗜胆菌属(bilophila);心脾两虚组优势菌属包括:鞘氨醇单胞菌属(sphingomonas)、甲基杆菌属(methylobacterium);阴虚火旺组优势菌属包括:拟杆菌属(bacteroides)、副拟杆菌属(parabacteroides)、parasutterella、butyricimonas、odoribacter。结论原发性失眠患者具有丰富的肠道菌群多样性及不同菌群结构,可能影响着不同中医证候的发生、发展及结局。
Objective To explore structural differences of intestinal flora in primary insomnia patients with different TCM syndromes through the high-throughput16S rDNA sequencing analysis.Methods Totally65patients with primary insomnia were divided into22patients with syndrome of liver depression transforming into fire,17patients with deficiency of both heart and spleen syndrome,26patients with syndrome of hyperactivity of fire due to yin deficiency,with47cases of healthy people as the control group.The fecal flora structure of the subjects was analyzed by high-throughput16S rDNA sequencing.QIIME software and R language stats package were used to analyze the diversity of flora.Results Totally1226different operational taxonomic units(OUTs)were obtained,and there were180significant differences among the4groups(P<0.05),indicating that the samples were rich in microbial colonies.The mapped reads in group of liver depression transforming into fire and hyperactivity of fire due to yin deficiency were more than the group of deficiency of both heart and spleen and the control group(P<0.05).Unweighted UniFrac analysis showed that the difference among groups was remarkably greater than the difference within group,and the grouping was statistically significant(R=0.103,P=0.002).It suggested that the diversity of intestinal flora was highly correlated with different TCM syndromes of insomnia.There were a total of57genera found significant differences among the different groups at the genus level(P<0.05),and115species at all species level.The dominant flora of the control group were prevotella,megamonas,clostridiumⅪ(clostridiumⅩⅧ),weissella,and alloprevotella;The dominant flora of liver depression transforming into fire syndrome were phascolarctobacterium,flavonifractor,eggerthella,and bilophila;The dominant flora of deficiency of both heart and spleen syndrome were sphingomonas and methylobacterium;The dominant flora in hyperactivity of fire due to yin deficiency syndrome group were bacteroides,parabacteroides,parasutterella,buty
作者
骆杰伟
吴永希
黄昉萌
陈炜玮
朱耀彬
魏世超
郑星宇
杨笑
LUO Jie-wei;WU Yong-xi;HUANG Fang-meng;CHEN Wei-wei;ZHU Yao-bin;WEI Shi-chao;ZHENG Xing-yu;YANG Xiao(Provincial Clinical Medical College, Fujian Medical University, Fuzhou 350001, China;Fujian Provincial Hospital, Fuzhou 350001, China;The Second Medical College, Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China;The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China;Department of Nursing, Fujian Health College, Fuzhou 350101, China)
出处
《中国中医药信息杂志》
CAS
CSCD
2018年第4期28-34,共7页
Chinese Journal of Information on Traditional Chinese Medicine
基金
福建省医药卫生科技创新项目(2015-CX-4)
福建省卫生系统中青年骨干人才培养项目资助计划(2015-ZQN-ZD-7)
福建省卫生计生青年科研课题(2016-1-84)
