摘要
基于3,5-二硝基水杨酸(3,5-dinitrosalicylic acid,DNS)比色法原理,利用了氧化钙和待测液反应形成的碱性环境及放出的热量,能够促使还原糖与DNS试剂发生显色反应的方法制备了还原糖快速检测试纸。实验对试纸的制备条件及测定范围进行研究,并制备了标准比色卡。实验结果表明,最佳基纸为快速定性滤纸,显色剂质量浓度为6.0 g/L,干燥温度为50℃。该试纸检测时间仅需5 min,检测范围为0.01~3.00 mol/L,具有操作简单、耗时短、显色迅速、携带方便等优点,适用于食品、发酵、医药等行业中对还原糖的快速半定量检测。
A rapid strip test for the detection of reducing sugar was developed based on the fact the chromogenic reaction of reducing sugar and3,5-dinitrosalicylic acid(DNS)is triggered by the alkaline environment and the heat generated by the reaction between calcium oxide and aqueous samples.The experimental conditions were determined and the detection range of the test system was investigated.Standard colorimetric card was prepared.The results showed that the best base paper was rapid qualitative filter paper,the concentration of chromogenic agent was6.0g/L and drying temperature was50℃.The test required only5min and the detection range was0.01–3.00mol/L.This method had the advantages of simple operation,time saving,rapid color development and convenience.It was suitable for fast semi-quantitative detection of reducing sugar in food and medicinal fields and fermentation industry.
作者
刘萧
马千里
杨仁党
LIU Xiao;MA Qianli;YANG Rendang(State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第6期298-301,共4页
Food Science
基金
浙江省农产品化学与生物加工技术重点实验室浙江省农业生物资源生化制造协同创新中心开放基金项目(2016KF0201)
中央高校基本科研业务费专项(x2qs-D2171020)
制浆造纸工程学科引智基地项目(2015B020241001)
