摘要
通过研究落新妇苷对胰脂肪酶的抑制活性,进一步探索其调节脂肪代谢的可能原因。结果表明,落新妇苷对胰脂肪酶有一定的抑制作用,半抑制率浓度为105μmol/L,最大抑制率为56.3%;且抑制效果随二者作用时间延长而增强。酶动力学特征研究表明,落新妇苷是胰脂肪酶非竞争性抑制剂,不改变其与底物的亲和程度,但使最大反应速度减小。荧光光谱滴定法表明落新妇苷会使胰脂肪酶内源荧光淬灭,并使其最大发射波长红移,二者结合常数lg Ka为5.50,结合位点数n为1.17。
In this paper,we studied the inhibitory activity of astilbin on pancreatic lipase. The results showed that astilbin had inhibitory effect on pancreatic lipase with IC50 of 105 μmol/L and maximum inhibition rate of 56. 3%. The inhibitory effect increased with the interaction time between astilbin and pancreatic lipase. Enzyme kinetics study showed that astilbin was a noncompetitive inhibitor of pancreatic lipase,which did not change its affinity with the substrate,but decreased its maximum reaction rate. The fluorescence spectra showed that astilbin could quench the endogenous fluorescence of pancreatic lipase,and made its maximum emission wavelength red shift. Their binding constant(lgK_a) was 5. 50 and the binding-site number was 1. 17.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2018年第2期172-175,181,共5页
Food and Fermentation Industries
基金
国家自然科学基金项目(31760461)
江西省教育厅基金项目(GJJ160416)
江西省天然产物与功能食品重点实验室开放基金项目(2015003)
关键词
落新妇苷
胰脂肪酶
抑制作用
astilbin
pancreatic lipase
inhibiting effect
