摘要
采用差示扫描微量热仪(Nano DSC)研究了人工合成的胰岛素连锁多肽区(insulin linkage polymorohism region,ILPR)DNA片段在钾离子溶液中形成的G-四链体的热力学性质.在40~100℃温区准确测量了G-四链体的熔融温度、融化焓和等压比热.结果表明,ILPR G-四链体的构象转变的熔融中点温度在(67.63±0.16)℃和(96.2±0.01)℃,两个分立组份的融化焓分别为(170.9±3.7)kJ/mol和(392.0±0.75)kJ/mol.
The thermodynamic properties of the insulin linkage polymorohism region(ILPR)G quadruplex in the presence of K+ions were measured by nano differential scanning calorimetry(Nano DSC)in solution.The melting temperature melting enthalpy and constant pressure specific heat of the G quadruplex were measured accurately in the range of40℃to110℃.The DSC thermogram of this sequence shows a melting curve with melting transitions of(67.63±0.02)℃and(96.2±0.02)℃,and the melting enthalpies are(170.9±2.1)kJ/mol and(392.0±1.6)kJ/mol.
作者
张又右
史全
王思雨
谭志诚
王韶旭
ZHANG Youyou;SHI Quan;WANG Siyu;TAN Zhicheng;WANG Shaoxu(School of Environmental and Chemical Engineering,Dalian Jiaotong University,Dalian 116028, China;Dalian Institute of Chemical Physics,Chinese Academy of Sciences, Dalian 116023,China)
出处
《大连交通大学学报》
CAS
2018年第1期100-104,共5页
Journal of Dalian Jiaotong University
基金
辽宁省自然科学基金资助项目(2015020211)
