摘要
目的观察2,5二羟基苯乙酮(DHAP)对多发性骨髓瘤(MM)细胞的影响,并探索其相关作用机制。方法体外培养RPMI8226细胞,分别加入浓度为100μM、200μM、400μM DHAP,对照组加入等量磷酸盐缓冲液(PBS)处理。48 h后收集各组细胞,利用流式细胞仪检测细胞凋亡情况。实时荧光PCR检测各组细胞Wnt/β-catenin信号通路相关基因β-catenin、Survivin、C-myc以及C-cyclin D1 mRNA表达。Western blotting检测β-catenin、Survivin、Cmyc以及C-cyclin D1蛋白表达。结果 48 h后对照组细胞凋亡率为(0.02±0.01)%,100μM、200μM、400μM DHAP组细胞凋亡率为(7.34±2.56)%、(12.18±3.07)%、(15.76±2.77)%,并呈剂量依赖趋势(P<0.05);实时荧光PCR结果显示,DHAP组β-catenin,Survivin、C-myc以及C-cyclin D1 mRNA表达显著低于对照组,并呈剂量依赖趋势(P<0.05);Western blot显示,DHAP组β-catenin、Survivin、C-myc以及C-cyclin D1蛋白表达显著低于对照组,并呈剂量依赖趋势(P<0.05)。结论 2,5二羟基苯乙酮呈剂量依赖性诱导多发性骨髓瘤细胞凋亡,可能与Wnt/β-catenin信号通路相关。
ObjectiveTo explore the effects of2,5-Dihydroxyacetophenone on multiple myeloma cells,and its underlying mechanism.MethodsThe experiment was divided into4groups,the control group(treated with PBS only),DHAP groups(treated with100,200,400μM DHAP,respectively).Apoptosis rate was detected by flow cytometry at48h after treatment.The mRNA expression ofβ-catenin,Survivin,C-myc and C-cyclin D1were detected by real time PCR and the protein expression ofβ-catenin,Survivin,C-myc and C-cyclin D1were valued by western blot.ResultsThe apoptosis rate of each group were(0.02±0.01)%,(7.34±2.56)%,(12.18±3.07)%,(15.76±2.77)%,the DHAP groups were significantly higher than that in the control group.Real time PCR indicated that the mRNA expression ofβ-catenin,Survivin,C-myc and C-cyclin D1in DHAP groups were markedly lower than that in the control group.What was more,the protein expression ofβ-catenin,Survivin,C-myc and C-cyclin D1in DHAP groups were also effectively lower than that in the control group.Conclusion2,5-Dihydroxyacetophenone can induce apoptosis of multiple myeloma cells probably via inhibiting Wnt/β-catenin signaling pathway.
作者
胡平
张霞
郭秀
郭毅刚
万楚成
HU Ping;ZHANG Xia;GUO Xiu(Department of Hematology, Taihe Hospital, Shiyan Hubei 442000, China.)
出处
《临床和实验医学杂志》
2017年第23期2338-2341,共4页
Journal of Clinical and Experimental Medicine
