摘要
-目的MicroRNA21表达在早期宫颈癌诊断中的应用价值。方法选取2015年3月~2016年3月本院行手术切除的43例宫颈癌患者为实验组,以同一时期行手术切除的21例宫颈上皮内瘤变的患者为对照组,从两组患者的组织标本中提出RNA,采用荧光PCR定量检测组织和细胞MicroRNA-21的表达,对结果进行分析。结果MicroRNA-21在宫颈癌患者中的表达,与年龄大小、肿瘤的直径及FIGO分期无相关性(P>0.05),与淋巴结是否转移及分化程度的高低有关(P<0.05);实验组患者组织标本中MicroRNA-21表达阳性率为86.0%,对照组患者组织标本中MicroRNA-21表达阳性率为38.1%,实验组高于对照组(P<0.01)。结论MicroRNA-21在宫颈癌患者中的表达,与其病情的发生发展有直接关系,可以为宫颈癌的早期诊断提供参考依据。
Objective To investigate the role of microRNA21in the diagnosis of early cervical cancer Methods A total of43cervical cancer patients underwent surgical resection were enrolled as the experimental group from March2015to March201621patients with cervical intraepithelial neoplasia were enrolled as the control group RNA was extracted from the tissue samples of the two groups,and the expression of microRNA21was detected by fluorescence PCR The results were compared between the two groups Results The expression of MicroRNA21in cervical cancer patients was not correlated with age,tumor diameter and FIGO staging(P>005),but correlated with the degree of metastasis and differentiation of lymph nodes(P<005)The positive rate of microRNA21in the experimental group was higher than the control group(860%vs381%,P<005)Conclusion The expression of MicroRNA21is related to the occurrence and development of cervical cancer,which can provide reference for the early diagnosis
作者
谢巧婷
XIE Qiao-ting(Department of Pathology,Anyang Maternal and Child Health Hospital,Anyang,Henan,455000,China)
出处
《临床研究》
2017年第12期67-68,共2页
Clinical Research
