摘要
目的研究胰岛素抵抗大鼠游泳运动干预后,血清炎性细胞因子IL-2、IL-6变化情况,可以为进一步减轻和防止糖尿病的发生、发展提供科学依据.方法采用8周龄雄性SD大鼠,制备腹腔胰岛素抵抗模型:实验鼠腹腔注射纯度为95%的牛Fetuin-A,剂量为0.75 mg/kg.8 h后检测,以胰岛素抵抗指数(HOMA-IR)高低来判定造模是否成功.实验设空白对照组(C组)以及造模成功的大鼠随机分为安静对照组(RR组)和运动组(RE组)2组,每组6只.RE组给予8周无负重游泳运动,60 min/d,C组、RR组在RE组运动期间浸水随即捞出.采用免疫组化和免疫印迹法分别检测血清Fetuin-A,胰岛素抵抗指数(HOMA-IR);采用ELISA检测血清IL-2、IL-2R、IL-6含量.结果 RE组HOMA-IR显著低于RR组.RE组较RR组大鼠血清IL-2水平明显升高(P<0.05);RR组与C组比血清IL-6含量升高幅度明显;RE组与RR组比大鼠血清IL-6含量降低(P<0.01).结论游泳运动降低胰岛素抵抗指数的同时,显著提高了IL-2,改善心脏舒张功能.而IL-6降低了表达,说明IL-6也可以作为检测胰岛素抵抗参考指标.
Objective To explore the changes of serum IL-2and IL-6of rats with insulin resistance afterswimming training intervention,so as to provide scientific basis for further reducing and preventing the occurrenceand development of diabetes.Methods Eight weeks male SD rats were induced to insulin resistance model byabdominal injection of0.75mg/kg of bovine Fetuin-A(purity of95%).The insulin resistance index(HOMA IR)8hours after injection was detected to confirm the success of modeling.The model rats were randomly divided intocontrol group(RR)and exercise group(RE),and normal rats were selected as the blank control group(groupC),6rats in each group.Rats in the RE group were given swimming training without weight loading,60min/d for8weeks,rats in group C and group RR were removed from the swimming pool immediately after flooding.Immunohistochemistry and western blot method were used respectively to detect the serumFetuin-A and the indexesof insulin resistance(HOMA IR).ELISA was used to detect serum IL-2,IL-2R and IL-6levels.ResultsThe HOMA-IR of rats in RE group was significantly lower than that in RR group.Compared with the RR group,the serum IL-2levels of rats in RE group were increased significantly(P<0.05).Compared with the C group,the serum IL-6levels in RR group were increased significantly.Compared with the RR group,the serum IL-6levels in RE group were decreased significantly(P<0.01).Conclusions Swimming training can significantlyimprove the IL-2levels and the diastolic function of heart at the same time of reducing insulin resistance index.Andthe down-regulation of IL-6expression suggests that IL-6may also be used as a reference to test insulin resistanceindex.
作者
汪君民
吴晓虹
王丽
雷德鸿
邱良武
WANG Jun-min;WU Xiao-hong;WANG Li;LEI De-hong;QIU Liang-wu(P.E. College of Nantong University,Jiangsu Nan tong 226019;Dept. of P.E. ,Kunming Medical University,Kunming Yunnan 650500,China;Journal Editorial Department,Kunming Yunnan 650500,China;Second Clinical Medical College,Kunming Yunnan 650500,China)
出处
《昆明医科大学学报》
CAS
2017年第4期18-21,共4页
Journal of Kunming Medical University
基金
福建省自然科学基金资助项目(2011J01151)
