摘要
研究利用大孔吸附树脂对水蜈蚣总多酚进行纯化的最佳工艺及其体外抗氧化性能。采用静态和动态吸附与解吸的方法对水蜈蚣提取液中的总多酚进行纯化,以吸附率和解吸率为指标,利用紫外分光光度计测量总多酚的含量。最佳树脂为DM-301型,最佳动态工艺条件:上样液体积25 mL,总多酚质量浓度为0.834 mg/mL,吸附流速2 BV/h,样品液pH值为2.0,解吸液乙醇体积分数为75%,洗脱液用量为30 mL,洗脱流速3 BV/h。纯化后浸膏中的总多酚纯度可达39.4%。该工艺操作简单,重复性好,适合水蜈蚣总多酚的纯化。考察提取液对DPPH、ABTS、超氧阴离子自由基的清除能力,并用Vc作为对比,结果表明,水蜈蚣总多酚的抗氧化能力与Vc的抗氧化能力接近。
The optimum purificaiton process and the antioxidant activity of the total polyphenols from Kyllinga brevifolia Rottb by use of macroporous resin were studied.The total polyphenols in Kyllinga brevifolia Rottb extract were purified by static and dynamic adsorption and desorption to calculate adsorption rate and desorption rate,and the content of the total polyphenols were determined by ultraviolet spectrophotometer.Results showed that the resin DM-301was most suitable for purification;the optimum dynamic conditions were as follows:loading amount25mL,the mass concentration of total polyphenols0.824mg/mL,absorption flow rate2BV/h,sample pH2.0,desorption solution(ethanol)volume fraction75%〇,eluent amount30mL,and eluent flow rate3BV/h.The purity of the total polyphenols in the purified extract reached39.4%〇.The process is simple in operation,repeatable,and suitable for purification of total polyphenols from Kyllinga brevifolia Rottb.The scavenging ability of total polyphenols to DPPH,ABTS and superoxide anion free radicals were studied by selecting Vc as contrast,and results showed that the antioxidant activity of the total polyphenols from Kyllinga brevifolia Rottb was similar to that of Vc.
作者
杨艳俊
王亚红
吉惠杰
YANG Yanjun;WANG Yahong;JI Huijie(School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022 , China)
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2017年第5期76-81,共6页
Journal of Henan University of Technology：Natural Science Edition
基金
吉林化工学院项目(吉化院合字[2015]第44号)
关键词
水蜈蚣
总多酚
纯化工艺
抗氧化
Kyllinga brevifolia Rottb
total polyphenol
purification
oxidation resistance
