摘要
目的分析南通汉族人群的基因表型,评测17个Y-STR基因座在南通人群中的应用价值。方法采集343名南通汉族男性无关个体的外周血样本,通过Chelex-100法提取基因组DNA,用Amp FlSTR Yfiler^(TM)试剂盒进行基因分型,并与12个汉族人群[安徽、江苏、江西、山东、上海、浙江(1)、兰州、南阳、泸州、牡丹江、山西和浙江(2)]以及9个少数民族人群(蒙古族、锡伯族、拉萨藏族、青海藏族、哈萨克族、维吾尔族、满族、台湾排湾族和土家族)进行比较。结果南通汉族群体在17个Y-STR基因座共检出327种单倍型,单倍型多样性(haplotype diversity,HD)值为0.999 7,与其他人群间的R_(st)值范围为-0.000 6~0.263 5。多维尺度图结果显示南通汉族人群与大多数汉族人群之间差异无统计学意义,但明显有别于其他少数民族人群。结论 17个Y-STR基因座在南通汉族人群中的群体多态性高,具有法医学应用价值。
Objective To analyze the genetic phenotypes of Nantong Han population and evaluate the application value of17Y-STR loci in Nantong population.Methods The peripheral blood samples were collected from343unrelated Nantong Han males and the genomic DNA were extracted by Chelex-100method.Genotyping was performed using the AmpF?詛STR YfilerTM Kit.The results were compared with other12Han populations,including Anhui,Jiangsu,Jiangxi,Shandong,Shanghai,Zhejiang(1),Lanzhou,Nanyang,Luzhou,Mudanjiang,Shanxi and Zhejiang(2),and9minority populations(Mongol,Xibe,Tibetan in Lhasa,Tibetan in Qinghai,Kazak,Uighur,Manchu,Paiwan in Taiwan and Tujia).Results A total of327different haplotypes were found in17Y-STR loci in Nantong Han population.The haplotype diversity(HD)was0.9997.The Rst value between Nantong Han and other Chinese populations ranged from-0.0006to0.2635.The multidimensional scaling results showed that Nantong Han population had no significant differences between most of the Han populations,but had significant differences between most of Chinese minority populations.Conclusion Seventeen Y-STR loci can be a powerful tool for forensic application because of its high polymorphism in Nantong Han population.
作者
杨敏
许思娴
秦海燕
张浩
伊海
韩海军
贾东涛
张玉红
李士林
YANG Min;XU Si-xian;QIN Hai-yan;ZHAGN Hao;YI Hai;HAN Hai-jun;JIA Dong-tao;ZHANG Yu-hong;LI Shi-lin(School of Life Science, Fudan University, Shanghai 200438, China;Nantong Municipal Public Security Bureau, Nantong 226007, China)
出处
《法医学杂志》
CAS
CSCD
2017年第4期383-387,共5页
Journal of Forensic Medicine
基金
国家自然科学基金资助项目(31271338)
教育部科学技术研究资助项目(113022A)
国家高技术研究发展计划资助项目(2012AA021802)
关键词
法医遗传学
多态现象
遗传
Y染色体
短串联重复序列
汉族
南通
forensic genetics
polymorphism, genetic
Y chromosome
short tandem repeat
Han population
Nantong
