摘要
目的:探讨脑白质损伤(WMD)早产儿脐血核转录因子-kB(NF-kB)与髓磷脂碱性蛋白(MBP)水平的早期变化及其与预后的关系。方法:选择WMD的早产儿,根据头颅核磁共振成像(MRI)将WMD组早产儿分为轻度组与中重度组。另选取同期30例正常早产儿作为对照组。采用酶联免疫吸附测定法(ELISA)检测所有早产儿脐血NF-kB和MBP蛋白表达水平。随访12个月后,采用Bayley婴幼儿发育量表对WMD早产儿的运动发育指数(PDI)和智能发育指数(MDI)进行评估。分析其核转录因子-kB(NF-kB)和MBP在不同严重程度WMD早产儿中的水平变化,及其与MDI和PDI评分的相关性。结论:与对照组比较,WMD组轻度组和中重度组NF-kB和MBP水平差异有统计学意义(P<0.05)。随着WMD程度的加重,NF-kB和MBP水平逐渐升高(P<0.05)。中重度组WMD早产儿MDI评分和PDI评分均显著低于轻度组(P<0.01)。NF-kB水平与MDI评分和PDI评分均呈现负相关(r=0.736,r=0.648,P<0.05)。MBP水平与MDI评分和PDI评分均呈负相关(r=0.625,r=0.842,P<0.05)。结论:WMD早产儿NF-kB和MBP水平显著升高,且随着WMD程度的加重,NF-kB和MBP水平越高;与MDI评分和PDI评分呈负相关,提示出生后较高的NF-kB和MBP预示不良的PDI和MDI。
Objective:To explore the early change of levels of umbilical cord blood nuclear factor-kB(NF-kB)and myelin bas-ic protein(MBP)in premature infants with white matter damage(WMD)and its relationship with infants,prognosis.Methods:The premature infants who were admitted and diagnosed with WMD were selected and divided into mild group and moderate-severe group ac-cording to cranial magnetic resonance imaging(MRI).Another30normal premature infants served as control group.Enzyme-linked im-munosorbent assay(ELISA)was used to detect the expression levels of NF-kB and MBP in the umbilical cord blood of premature in-fants.All infants were followed up for12months,and Bayley Scales of Infant and Toddler Development was applied to evaluate the psy-chomotive development index(PDI)and mental development index(MDI)of premature infants with WMD.The changes of the levels of NF-kB and MBP in premature infants with different-degree WMD as well as their correlation with MDI and PDI scores were analyzed.Results:Compared with control group,there were significant differences between mild group and moderate-severe group in the levels of NF-kB and MBP(P<0.05).The levels of NF-kB and MBP increased along with the aggravation of WMD(P<0.05).Moderate-se-vere group was evidently lower than mild group in MDI and PDI scores(P<0.01).NF-kB level was in negative association with MDI and PDI scores(r=0.736,r=0.648,P<0.05).Moreover,MBP level was also negatively associated with MDI and PDI scores(r=0.625,r=0.842,P<0.05).Conclusion:The levels of NF-kB and MBP increase notably in premature infants with WMD.The severer the WMD is,the higher the levels are.Both NF-kB and MBP are in negative association with MDI and PDI scores,indicating that higher levels of NF-kB and MBP may suggest poor PDI and MDI after infants,delivery.
作者
王智英
霍海花
吕红艳
栗庆芳
WANG Zhi-ying;HUO Hai-hua;LV Hong-yan;LI Qing-fang(Department of Neonatology Handan Maternity and Child Care Center;Department of Pediatrics yCixian People’s Hospital ,Handan 056000, Hebei China)
出处
《川北医学院学报》
CAS
2017年第4期518-521,共4页
Journal of North Sichuan Medical College
基金
河北省医学科学研究重点课题计划(20150445)
