摘要
目的:探讨开心散对阿尔茨海默病大鼠学习记忆能力的影响。方法:清洁级Wistar大鼠60只,随机分为5组,空白组、模型组、开心散低剂量组、开心散中剂量组、开心散高剂量组,每组12只,采用Aβ1-42寡聚体注射大鼠双侧海马区进行造模,造模成功后进行4周的治疗,开心散组给予开心散低、中、高剂量灌胃治疗,空白组及模型组给予等量蒸馏水灌胃。各组经过治疗后,记录其逃避潜伏期、第2象限时间百分比、穿越次数的变化。结果:自定位航行实验第2天开始,开心散各剂量组逃避潜伏期均显著缩短,高剂量组下降趋势明显快于中、低剂量组,差异有统计学意义(P<0.05);第2象限时间百分比及穿越次数变化中,开心散各剂量组均有提高,但开心散高剂量组提高幅度较中、低剂量组显著,差异有统计学意义(P<0.05),低剂量组与中剂量组相比无显著差异(P>0.05)。结论:开心散能够有效提高大鼠学习记忆能力,且高剂量开心散疗效最优。
Objective:To explore effects of Kaixin San on learning and memory ability of Alzheimer’s disease in rats.Methods:60rats were randomly divided into five groups,including blank group,model group,KP low-dose group,KP mid-dose group and KP highdosegroup,12cases in each group.Models were established by injecting Aβ1-42oligomer into bilateral hippocampus.After models weresuccessfully established,the KP groups were given different doses of Kaixin San.The other two groups received the same volume distilledwater.After four-week-treatment,the changes of escaping latency,percentage of the second quadrant time and traversing times were observed.Results:In the second day of orientation navigation experiments,the escaping latency was significantly shorter in the KP groups.The downward trend was more obvious in the high-dose group,with significant differences(P<0.05).The second quadrant time and traversingtimes showed marked increasing in the KP groups.The high-dose group has a higher trend than the low-dose group and mid-dose group(P<0.05),with no statistical difference(P>0.05).Conclusion:Kaixin San has a marked effect on ameliorating learning and memory abilityof Alzheimer's disease in rats.The high-dose has the best effect.
出处
《中医临床研究》
2017年第10期44-46,共3页
Clinical Journal Of Chinese Medicine
关键词
开心散
阿尔茨海默病
学习记忆能力
Kaixin San
Alzheimer's disease
Learning and memory ability
