摘要
FAR-RED ELONGATED HYPOCOTYL 3(FHY3)和它的同源基因FAR-RED-IMPAIREDRESPONSE 1(FAR1)参与远红光、脱落酸(Abscisic acid,ABA)对拟南芥种子休眠的调节,然而FHY3/FAR1在木本植物花芽休眠中的表达尚不清楚。本研究以八年生‘中油四号’油桃花芽为材料,利用NCBI找出桃树上的FAR1/FHY3同源基因,测定了同源性最高的5个基因在休眠诱导期的表达,并在休眠诱导期分别用脱落酸及远红光处理桃树,荧光定量检测了基因的表达量变化。结果表明,桃中同源性最高的Prupe.3G186100、Prupe.3G186200、Prupe.4G115100、Prupe.3G186000 4个基因在休眠诱导期均有上调,且受ABA诱导表达上调,受远红光诱导表达下调,而Prupe.2G327900在休眠诱导期表达下调,受ABA诱导下调,受远红光诱导上调。我们推测,Pp FRSs基因可能通过不同的模式参与油桃花芽休眠诱导的调控。
FAR-RED ELONGATED HYPOCOTYL3(FHY3)and its homolog FAR-RED IMPAIRED RESPONSE1(FAR1),are involved in the regulation of seed germination induced by far red and abscisic acid,but the transcriptional changes of FHY3/FAR1in dormancy induction process of flower bud is still to be explored.The homologous genes of Arabidopsist haliana in peach were firstly identified by NCBI blast toolkit.At the same time the expression pattern of the five highesthomology genes was characterized during the dormancy induction course,far-red light conditions and ABA-treated conditions,respectively,in floral buds of seven-year-old‘Zhongyousihao’nectarine(Prunus persica var.nectariana cv.‘Zhongyousihao’).Quantitative real-time PCR analysis demonstrated that the expression of Prupe.3G186100,Prupe.3G186200,Prupe.4G115100,and Prupe.3G186000have a up-regulated tendency during the dormancy induction course,also,their transcripts are up-regulated by ABA,down-regulated by far-red light.Conversely,Prupe.2G327900transcript isdown-regulated during both the dormancy induction course and ABA-treated conditions,but up-regulated by far-red light.According to the aforementioned results,we could speculate that PpFRSs may be involved in dormancy induction of flower bud through different methods.
作者
郇蕾
王旭旭
刘文海
李玲
高东升
HUAN Lei;WANG Xu-xu;LIU Wen-hai;LI Ling;GAO Dong-sheng(College of Horticulture Science and Engineering/Shandong Agricultural University, Tai’an 271018, China;State Key Laboratory of Crop Biology/Shandong Agricultural University, Tai’an 271018, China;Weifang Engineering Vocational College, Weifang 261000, China)
出处
《山东农业大学学报（自然科学版）》
CSCD
2017年第2期229-235,共7页
Journal of Shandong Agricultural University：Natural Science Edition
基金
山东省自然科学基金(NO.ZR2014CM015)
