摘要
考察热处理时间对罗非鱼皮酸溶性胶原(acid-soluble collagen,ASC)理化性质和成膜能力的影响。结果发现,热处理时间对胶原的紫外吸收和分子质量分布没有明显影响;随着热处理时间的延长,ASC的比浓黏度发生下降,变性温度和热转变温度分别从32.6℃和38.15℃下降至27.0℃和29.19℃。浊度实验结果表明,当ASC在42℃条件下热处理超过10 min后,ASC不具备成纤维能力。而且,热变性的胶原还会阻碍天然胶原的纤维形成。另一方面,随着热处理时间的延长,胶原膜的抗拉伸强度从30.81 MPa增加至41.33 MPa,断裂延伸率从8.95%增加至11.59%,透明度值从2.42增加至3.33。根据扫描电子显微镜观察的结果,发现胶原膜的纤维状网络结构随着热处理逐渐转变成类似皱纹的纹理结构。以上研究结果表明,经过热变性后,罗非鱼皮ASC的成纤维能力下降,但成膜能力却出现上升。
The effect of thermal treatment time on physicochemical properties and film-forming ability of acid-soluble collagen(ASC)from tilapia skin was investigated.No significant difference in UV absorption or molecular weight distribution of ASC was observed during thermal treatment.With increasing thermal treatment time,the specific viscosity of ASC was decreased,and the denaturation temperature and thermal transition temperature were decreased from32.6to27.0℃and from38.15to29.19℃,respectively.When thermal treatment time was more than10min at42℃,The results of turbidity experiments indicated that the ASC did not possess fibril-forming ability after10min treatment at42℃.Furthermore,the fibril formation of natural collagen was also hindered by thermally denatured collagen.On the otherh and,the tensile strength of collagen films was increased from30.81to41.33MPa,the elongation at breaks from8.95%to11.59%,and the transparency value from2.42to3.33with increasing thermal treatment time.Based on the results ofscanning electron microscopy,it was found that the fibrous structure of collagen films was gradually converted to a wrinklelike structure during thermal treatment.From the above results,it could be concluded that the fibril-forming ability of ASC from tilapia skins was decreased after thermal denaturation,while the film-forming ability was increased.
作者
张琳琳
陈俊
翁武银
ZHANG Linlin;CHEN Jun;WENG Wuyin(College of Food and Biological Engineering, Jimei University, Xiamen 361021, China;Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第5期80-85,共6页
Food Science
基金
国家自然科学基金面上项目(31271984
31571835)
福建省杰出青年科学基金项目(2014J06013)
厦门市海洋经济发展专项资金项目(14CZP031HJ05)
福建省海洋经济创新发展区域示范项目(2014FJ05)
关键词
酸溶性胶原
热处理
成纤维能力
成膜能力
罗非鱼皮
acid-soluble collagen
thermal treatment
fibril-forming ability
film-forming ability
tilapia skin
