摘要
目的探讨阻塞性睡眠呼吸暂停综合征(OSAS)模式间歇低氧(IH)对肺癌细胞系A549细胞YES相关蛋白(YAP)及磷酸化YAP(P-YAP)表达的影响。方法 IH分别处理A549细胞1 h、3 h、6 h(IH1组、IH3组、IH6组),IH频率为:5%O2300 s,21%O2300 s,并设置常氧对照组(N组)。q RT-PCR检测YAP m RNA表达;Western blot检测YAP及P-YAP蛋白表达。结果 IH1组(2.50±0.18)、IH3组(4.07±0.25)、IH6组(9.18±0.58)YAP m RNA的相对表达量显著高于N组(1.00±0.01),差异有统计学意义(均P<0.05),IH1组、IH3组、IH6组YAP蛋白的表达量较N组上调,并随暴露时间增加而上调;IH1组、IH3组、IH6组P-YAP蛋白的表达量较N组下调,并随暴露时间增加而下调。结论 YAP信号通路可能在OSAS模式IH促进肿瘤发生发展的过程中起重要的调控作用。
Objective To explore the effect of obstructive sleep apnea syndrome (OSAS), OSAS-like intermittent hypoxia (IH)on the expression levels of P -YAP and YAP in A549 lung cancer cell lines. Methods A549 cells were treated with IH exposure ( exposed to 5% O2 for 300 seconds and 21% O2 for 300 seconds) for 1, 3 and 6 h (IH1, IH3, IH6) or normoxia exposure (N group). Quantificational real-time PCR was used to measure the mRNA expression levels of YAP.Western blot assay was used to detect the protein expression levels of YAP and P-YAP. Results The mRNA expression levels of YAP were significantly increased with the increase of IH exposure time points in IH1 (2.50±0.18), IH3 (4.07±0.25)and IH6 (9.18±0.58) groups than those in N group (1.00±0.01) (all P<0.05). The protein expression levels of YAP were significantly increased with the increase of IH exposure time points in IH1, IH3 and IH6 groups than those in N group. The protein expression levels of P-YAP were significantly decreased with the increase of IH exposure time points in IH1, IH3and IH6 groups than those in N group. Conclusion YAP cell signaling plays an important role in the process of OSAS-like IH induced tumor development.
作者
薛震
李莲
任方元
姜芳馨
陈宝元
曹洁
XUE Zhen;LI Lian;REN Fangyuan;CHEN Baoyuan;CAO Jie;JIANG Fangxin(Department of Respiratory Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China;College of Life Sciences, Nankai University)
出处
《天津医药》
CAS
2016年第12期1414-1417,共4页
Tianjin Medical Journal
基金
国家自然科学基金资助项目(81500070
81670084)
天津医科大学总医院青年孵育基金项目(ZYYFY2014011)
