摘要
离体条件下风信子(Hyacinthus oricntalis L.) 再生雄蕊的小孢子发生和雄配子体发育的细胞学观察表明:从四分体到二细胞花粉,二者的细胞学特点基本一致。发育到成熟花粉时,再生雄蕊的成熟花粉是三细胞的,自然情况下是二细胞的。表明了两者在生殖细胞分裂完成的时间和完成时在细胞中所处的位置等方面都存在明显的差异。对这种差异产生的原因及是否会对精细胞产生影响等方面进行了讨论。
The present study consists of the cytological observations on the process of microsporo- genesis and pollen development in the regenerated stamen of hyacinth; and a comparative study of the cytological changes in stamens of both regenerated and produced under natural condi- tion. Results showed that the cytological changes of microsporogenesis and the pollen develop- ment in the regenerated stamen of the hyacinth were basically normal. But in the stage of the mature pollen there was an obvious cytological difference between both stamens in vitro and in nature. The mature pollen of the regenerated stamen consisted of three cells: one vegetative cell and two sperms, while mature pollen grain under natural condition was made up of two cells: one vegetative cell and one generative cell. This difference mainly resulted from different time and place of the generative cell division. The reason resulting in the differences and their influence on sperms were discussed.
基金
国家自然科学基金
关键词
风信子
小孢子发生
花粉发育
Hyacinthus orientalis L.
Microsporogenesis
Pollen development