摘要
目的 : 研究螺旋藻多糖 (PSP)对固定化藻蓝蛋白 (PC)抑癌作用的影响。方法 : 钝顶螺旋藻经热水抽提 ,脱蛋白 ,醇沉淀得粗多糖 PSP,PSP经 DEAE-Sephadex柱层析得到组分多糖 PSP 和 PSP 。将 PSP 溶液添加在接枝有 PC的组织培养聚苯乙烯培养孔内 ,对人胰腺癌SW1 990细胞进行无血清培养 48h。结果 : PSP浓度为 1 5 mg/L时 ,以多糖对癌细胞生长的抑制作用为主 ,而在 1 0 ,1 7.5 ,2 5 mg/L浓度时均以固定化 PC的抑制作用为主 ,多糖有一定的协同作用。固定化 PC浓度以 0 .0 2 mg/well为最佳浓度 ,对胰腺癌 SW1 990细胞的抑制作用最强 ,在 1 7.5mg/L PSP存在下 ,抑制率最高可达到 86%。结论 : 表面吸附 PSP可以进一步提高接枝
Objective: To investigate the influence of polysaccharide of Spirulina platensis(PSP) on inhibition activity of immobilized phycocyanin (PC) against proliferation of pancreatic cancer cells SW1990. Methods: PSP was added to the wells of cell culture polystyrene which was immobilized with PC, and SW1990 cells were cultured on this kind of biomaterial in serum free medium. Results: When the concentration of PSP was 15 mg/L, the inhibition activity was mainly caused by PSP, and when the concentrations were 10, 17.5 and 25 mg/L, PSP was cooperative with immobilized PC. The best immobilized amount of PC was 0.02 mg/well, which could inhibit 86% SW1990 cells. Conclusion: PSP can enhance the inhibition activity against pancreatic cancer cells SW1990 from immobilized PC.
出处
《营养学报》
CAS
CSCD
北大核心
2002年第3期252-255,共4页
Acta Nutrimenta Sinica
基金
广东省自然科学基金 ( N o.970 84 8)
