摘要
以云南普通野生稻基因组DNA为模板,根据GeneBank中登记的编码小麦高亲和力磷酸转运蛋白基因保守序列设计一对特异引物,利用多聚酶链反应技术(PCR),扩增出目标基因(cwrpt)1002bp长度的基因片段并克隆到载体pGEM T.经DIG标记探针杂交检测初筛、限制性内切酶酶切、PCR扩增、DNA序列分析与可能氨基酸序列同源性比较,此推定的氨基酸序列与小麦、水稻、拟南芥、番茄磷酸盐转运蛋白同源性很高,初步认为此基因片段为普通野生稻耐低磷胁迫相关磷酸盐转运蛋白的编码基因,获得全长序列与基因表达的进一步研究正在进行中.
A pair of specific primers was designed according to the gene sequence encoding high affinity phosphate transport protein for wheat adopted in GeneBank. By the improved PCR amplification technology, special cDNA of 1 002 bp nucleotide was obtained from common wild rice of Yunnan and named cwrpt. And this cDNA nucleotide sequence has high homology with that of the phosphate transport protein genes coding wheat, rice, tomato and Aribidopsis thaliana. It was suggested that cDNA fragment is related with phosphate transport protein in common wild rice of Yunnan. The total length of this cDNA and related gene expression is still carried on.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2002年第5期588-592,共5页
Journal of Fudan University：Natural Science
基金
国家博士后基金资助(中博基(中)200 31号)
遗传学科创新性基础研究资助项目
