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PKCε与Lck在细胞内直接相互作用的荧光共振能量转移证据 被引量：1

Fluorescence Resonance Energy Transfer Evidence of Direct Interaction Between PKCε and Lck in vivo
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摘要 蛋白激酶Cε亚型 (PKCε)和蛋白酪氨酸激酸Lck均在心肌缺血预适应的信号转导中起着十分重要的作用 .构建PKCε和Lck与绿色荧光蛋白的融合蛋白的真核细胞表达载体 .将其共转染H2 93细胞进行表达 .用荧光显微镜观察到荧光共振能量转移现象 。 Protein kinase Cε(PKCε) and tyrosine kinase Lck play critical roles in intracellular signal transduction of ischemic preconditioning of cardiomyocytes. The eukaryotic expression vectors of fusion proteins of PKCε and Lck with green fluorescent proteins were constructed and cotransfected into H293 cells for expression. Fluorescence resonance energy transfer phenomena in the cotransfected cells were observed with epi-fluorescence microscope which evidence a direct interaction between PKCε and Lck in vivo.
作者 曹西南 平佩佩 宋昌旭
机构地区 昆明医学院分子生物学实验室 DepartmentofPhysiologyandBiophysics
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2002年第5期529-534,共6页 Chinese Journal of Biochemistry and Molecular Biology
关键词 PKCΕ LCK 直接相互作用 荧光共振能量转移 缺血预适应 蛋白质相互作用 心肌缺血 HELA细胞 ischemic preconditioning,PKCε,Lck,protein interaction, fluorescence resonance energy transfer
分类号 R542.2 [医药卫生—心血管疾病] Q26 [医药卫生—内科学]
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参考文献13

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同被引文献23

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中国生物化学与分子生物学报
2002年 第5期

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