摘要
目的了解TTV山东分离株感染状况及基因变异的情况。方法应用逆转录聚合酶链反应(PCR)检测26例来自山东泰安、莱芜、济宁、菏泽、枣庄及临沂、日照地区非甲～庚型肝炎和12例肝细胞癌患者,其中HBV感染者5例,HCV感染者2例,HBV+HCV+HGV混合感染2例,HBV+HCV+TTV感染者1例,HGV+TTV感染者1例,HEV+HGV感染者1例。在这26例非甲～非庚型肝炎中有输血或输血制品、手术、拔牙、针灸、注射以及肝炎密切接触史17例;另12例肝癌中有输血、手术、拔牙、静脉注射史10例。检出血清中TTVDNA阳性扩增的产物利用PCR技术片段直接克隆和测序,分析其基因变异的情况。结果 26例非甲～庚型肝炎中11例TTVDNA阳性(42.3％)。对其中两株(TTVSD4、TTVSD5)部分基因克隆测序,并与日本株(ABOO8394)相比较,其核苷酸序列同源性分别为99.9％和100％。而12例肝细胞癌患者中3例TTVDNA阳性(25.0％),对其中一株(TTVSD6)部分基因克隆与测序,与日本株(ABOO8394)相比较,其核苷酸序列同源性为99％;TTV山东株三株间核苷酸同源性均为99％。结论本项研究证实山东地区非甲～庚型肝炎和肝细胞癌患者中存在着较高TTV感染,TTV感染可能具有嗜肝性,而且可能与肝功能损害有关,是引起非甲～庚型肝炎重要病原。
Objective To understand the
prevalence of TTV infection and genetic variation of
TTV isolated in Shandong Province. Methods TTV
DNA were amplified and detected by PCR from sera
of patients with NonA - G hepatitis and hepatic can-
cer. The PCR products were cloned and sequenced.
Results TTV DNA was positive in 11 of 26 patients
(42. 3% ) with NonA - G hepatits. Partial gene of
two TTV isolated (TTVSD4, TTVSD5 ) was
cloned, sequenced and compared with known se-
quence of TTV isolates in Japan (ABOO8394) re-
ported by Okamoto. The nucleotide homology were
99% and 100%. TTV DNA was postive in 3 of 12
patients (25 % ) with hepatic cancer. Partial gene of a
TTV isolated (TTVSD6) was cloned, sequenced and
compared with known sequence of TTV isolated in
Japan (ABOO8394) reported by Okamoto. The nu-
cleotide homology was 99%. Conclusion The ratio
of TTV infection is high in Shandong Province. TTV
might be hepatotropic, and it probably correlated
with the impairment of liver function. So it is likely
to be the pathogen of NonA～ G hepatitis.
出处
《传染病信息》
2002年第3期131-131,共1页
Infectious Disease Information
关键词
输血传播病毒
非甲-庚型肝炎
核苷酸序列
Transfusion transmitted virus
Non A～G hepatitis
Nucleotide sequence
