摘要
目的 研究人类端粒酶反义脱氧核苷酸及其与梁金菇多糖协同作用对HL 6 0细胞凋亡及端粒酶活性的影响。方法 端粒酶反义脱氧核苷酸单独或与梁金菇多糖协同处理HL 6 0细胞后 ,分别用涂片法、流式细胞仪法和电镜检测两种药物处理后对细胞凋亡的影响 ,以TRAP Elisa法检测对端粒酶活性的影响。结果 端粒酶反义核酸单独或与梁金菇多糖协同处理HL 6 0均可提高细胞的凋亡率并抑制端粒酶活性 ,两者协同处理效果更显著。结论 端粒酶反义核酸可降低细胞的端粒酶活性并诱导细胞调亡 ,梁金菇多糖则可加强这种作用。
Objective To study the synergy effect of telomerase antisense oligodeoxynucleotide and Liangjin mushroom on apoptosis and telomerase activity of HL-60 cells.Methods HL-60 cells were treated by telomerase antisense oligodeoxynucleotide singularly or synergically with Liangjin mushroom polysaccharides .Apoptosis was studied with cell culture smears, flow cytometer analysis and electron microscope. Telomerase activity was measured with TRAP-ELISA. Results Telomerase antisense oligodeoxynucleotide treated HL-60 cells with or without the polysaccharides could increase cell apoptosis rate and decrease telomerase activity, synergy of the two got more effective results. Conclusion Telomerase antisense oligodeoxynucleotide can decrease the telomerase activity and induce HL-60 cells apoptosis while Liangjin mushroom polysaccharides can enhance the effects.
出处
《肿瘤》
CAS
CSCD
北大核心
2002年第5期368-370,共3页
Tumor
