摘要
目的 观察神经性膀胱逼尿肌平滑肌细胞的凋亡现象 ,凋亡相关基因Bcl 2与bax的表达及二者之间的关系。方法 分别应用末端脱氧核糖核酸转移酶 (TUNEL)法、免疫组化方法 ,观察2 1例神经性膀胱和 12例正常膀胱逼尿肌平滑肌细胞凋亡和凋亡相关基因bcl 2和bax的表达。结果 病例组逼尿肌中bcl 2的阳性表达率和高表达率 (80 .9% ,33.3% )与正常组 (75 .0 % ,41.7% )相比无明显变化 (χ2 =1.2 ,P >0 .0 5 )。病例组逼尿肌中bax的阳性表达率和高表达率 (90 .5 % ,5 7.1% )较正常组 (6 6 .7% ,16 .7% )明显增加 (χ2 =7.6 ,P <0 .0 5 )。病例组平滑肌细胞凋亡指数为 9.4± 2 .5 /HP ,较正常对照组 4.7± 1.2 /HP明显增加 (P <0 .0 1) ,Spearman秩相关分析表明 ,凋亡指数与bcl 2的表达成负相关 (r =- 0 .6 ,P <0 .0 1) ,与bax表达成正相关 (r =0 .5 ,P <0 .0 5 )。结论 神经性膀胱逼尿肌平滑肌细胞凋亡较正常逼尿肌增加 ,Bcl 2 /bax参与凋亡的调控 ,bax表达增加 ,Bcl 2与bax之间的平衡破坏 ,是导致逼尿肌平滑肌细胞凋亡的主要因素。
Objective To observe the apoptosis in smooth muscle cell of detrusor in neurogenic bladder and expression of bcl-2 and bax as well as the relation between apoptosis and bcl-2/bax.Methods Terminal deoxyribonucleotidyl transferase mediated dUTP nick end labeling(TUNEL) and immunohistochemical staining were used to observe apoptosis of smooth muscle cell of detrusor and expression of bcl-2 and bax in 21 children with neurogenic bladder and 12 normal children. Results The expression rates and high expression rates of bcl-2 had no difference(χ 2= 1.2,P< 0.05) between neurgenic bladder ( 80.9%, 33.3%) and the normal ( 75.0%, 41.7%). But The expression rates and high expression rates of bax in detrusor of neurogenic bladder ( 90.5%, 57.1%) were higher (χ 2= 7.6,P< 0.05) than normal bladder( 66.7%, 16.7%). Apoptosis index( 9.4± 2.5/HP) of smooth muscle cell of neurogenic bladder increased (P< 0.01) comparing with normal detrusor( 4.7± 1.2/HP). Spearman correlation indicated that there was a negative relation between apoptosis index and expression of bcl-2 (r= -0.6,P< 0.01), but a positive relation between apoptosis index and expression of bax (r= 0.5, P< 0.05) in neurogenic bladder. Conclusions As compare with the normal bladder, apoptosis of smooth muscle cell of detrusor in neurogenic bladder is increased. Bcl-2/bax regulates apoptosis. Increase of expression of bax, and unbalance between bcl-2 and bax were the main causes of apoptosis of smooth muscle cell of detrusor. Apoptosis of smooth muscle cell of detrusor may be related to dysfunction of detrusor of neurogenic bladder.
出处
《中华小儿外科杂志》
CSCD
北大核心
2002年第5期451-453,共3页
Chinese Journal of Pediatric Surgery
