摘要
从自然环境中分离、筛选出了降解橡单宁能力较强的菌株 ,并对该菌株降解单宁的培养条件进行分析。结果表明 ,2 8～ 32℃、pH 4.5培养 8d为橡单宁的最适降解条件。在此条件下 ,对该菌株驯化 ,有效提高了该菌株的耐单宁性及单宁降解率。通过研究外加碳源、氮源及碳氮比对该菌株降解单宁的影响 ,发现这 3个因素均明显影响微生物对单宁的降解。在适宜的培养条件下 ,该降解菌在以葡萄糖为外加碳源 ,以硝酸铵为氮源 ,碳氮比为 1 0∶1的条件下 ,单宁的降解率最高 ,并发现在一定范围内 ,碳氮比高有利于菌株降解单宁 ,而碳氮比低则不利于单宁的降解。
Strain with valonia tannin resisting was isolated from nature and proved to be quite effective on biodegradation of valonia tannin.The optimum conditions for the degradation were pH 4.5 and 28 ℃ in 120 r/min for 8 days,which led to 64% COD removal rate of culture medium.The degradation rate of valonia tannin in culture medium was significantly increased by acclimatizing the strain.The effects of carbon and nitrogen sources and the ratio of carbon to nitrogen on biodegradation of valonia tannin were also investigated.Glucose takes advantage of the degradation.The highest biodegradation rate of valonia tannin in culture medium was achieved when the ratio of carbon and nitrogen was 10∶1 .It was also found that the higher ratio of carbon and nitrogen is more suitable for biodegradation of valonia tannin,and the higher nitrogen content obviously inhibits the degradation.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2002年第5期57-60,共4页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金资助项目 (2 9872 0 2 5 )
