摘要
采用分子生物学方法对小盾纤恙螨的媒介意义进行研究。结果表明：用ＲＴＰＣＲ和ＮｅｓｔｅｄＲＴＰＣＲ从恙螨体内检测到汉滩病毒ＲＮＡ；用原位分子杂交技术在恙螨的卵巢细胞、支肠细胞等组织中检测到汉滩病毒ＲＮＡ。以上结果为恙螨的媒介意义提供了具有分子水平的直接证据，对ＨＦＲＳ的流行病学和预防具有重要的理论意义和实用价值。
Chigger mite had been studied as a vector by molecular biological methods since 1994. The results show that HFRSV RNA could be detected by RT PCR and Nested RT PCR in chigger mity.TCID50/ml titer of HFRSV in larva and nymph detection demonstratied HFRSV could be transmitted by trans stadial and prolificated in chigger mite.HFRSV RNA located in ovary cells and midgut cells of chigger mite.The above results indicated that chigger mite is atransmitting vector of HFRSV.
基金
国家自然科学基金
