摘要
梯度蔗糖预培养能增强黄皮胚轴的脱水耐性,使胚轴含水量下降至17 6%仍保持活力,在含NAA和IAA的WPM培养基上可诱导植株再生。黄皮胚轴脱水至18%后分别采用直接冻存法、抗冻剂冻存法、微滴冻法和玻璃化法超低温保存都不能复活。玻璃化冻存后的胚芽超微结构基本完整,若能继续优化冻存和培养条件,有希望获得存活的胚芽及其再生植株。
Pretreatment with gradient sucrose significantly enhanced desiccation-tolerance of wampee axes which could survival under the moisture of 17.6%. Plantlets could be regenerated from the dried axes on woody plant medium contented hormones of α-napthaleneacetic acid and indole-3-butyric acid. These axes with moisture content of 18% could not survival liquid nitrogen(-196 ℃) even if by using cryoprotectants, droplet freezing and vitrification. However, substructure of plumule were almost intact during cryopreservation of the axes by vitrificating. The present technique of cryopreservation and reculture should by improved for the successful cryopreservation of wampee axes.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第S2期231-235,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
广东省自然科学基金资助项目(980360)
关键词
黄皮胚轴
梯度蔗糖
脱水耐性诱导
超低温保存
wampee axes
gradient sucrose
induction of desiccation-tolerance
cryopreservation
