摘要
苯酚-硫酸法是测定多糖的常用方法,影响该法显色的因素较多,对不同多糖物质,其最佳显色条件不同。采用正交试验设计法确定牛肝菌多糖的最佳显色条件。结果表明,在显色时间25m in、显色温度70℃、苯酚1.2mL、硫酸8.0mL条件下,采用苯酚-硫酸法测定牛肝菌多糖含量时,平均回收率达99.0%,RSD3.70%(n=6)。
Phenol-sulfuric acid method is a commonly used method for measuring polysaccharides.However,many factors affect the colored conditions and different types of polysaccharides have different colored conditions.In this test,colorimetric method for the determination of polysaccharides in boletus was developed by the use of phenol-sulfuric acid and optimized by orthogonal experimentations.The results showed that under the optimal conditions of time 25 min,temperature 70℃,phenol 1.2 ml and sulfuric acid 8.0 ml,this method was rapid and accurate with good reproducibility.The average recovery was 99.0﹪,and the relative standard deviation was 3.70﹪.
出处
《中国酿造》
CAS
北大核心
2008年第11X期86-88,共3页
China Brewing
关键词
牛肝菌
多糖
苯酚-硫酸法
含量测定
显色条件
boletus
polysaccharides
phenol-sulfuric acid method
content determining
colored conditions
