摘要
目的 探讨耳蜗 2～ 4回外毛细胞 (outerhaircell,OHC)L型钙通道分布密度。方法 豚鼠耳蜗外毛细胞L型钙通道经DM BODIPYDHP和RH414标记后用激光扫描共聚焦显微镜观察 ,以DM BODIPYDHP /RH414荧光比值大小指示L型钙通道密度的高低。结果 ①应用硝苯地平阻断后 ,质膜的DM BODIPYDHP荧光染色明显减弱 ,表明其钙通道为特异性L型钙通道 ;②L型钙通道密度从低频区 (第 4回 )向高频区 (第 2回 )有逐渐增加的趋势 ,方差分析示第 4回和第 2回OHC之间L型钙通道的分布差异有显著性意义 ;③OHC质膜不同区域L型钙通道分布差异无显著性意义。结论耳蜗 2～
Objective To probe the relationship between the density of L-type calcium channels on outer hair cells(OHCs) among various turns and the tonotopic map in the guinea pig cochlea. Methods The L-type calcium channels in outer hair cells of guinea pigs were labeled with DM-BODIPY DHP and RH414 and monitored using a laser scanning confocal microscope. The fluorescence ratio of DM-BODIPY DHP / RH414 revealed the distribution of L-type calcium channels on the cellular membrane. Results The results showed that the cellular membrane had lesser DM-BODIPY DHP staining when pretreated with nifedipine, indicating that calcium channels on OHCs had the specificity of a L-type calcium channel. The density of L-type calcium channels had a tendency of increasing gradually from low frequency areas to high frequency areas.Variance analysis showed that the difference of the density of L-type calcium channels on OHCs between the fourth turn and the second turn was significant. There were no apparent differences in the distribution of L-type calcium channels among various regions on the cellular membrane of OHCs. Conclusions The descripency of the distribution of L-type calcium channels on OHCs among various turns of the guinea pig cochlea may be one of the molecular mechanisms of the tonotopic map of the cochlea.
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2002年第5期321-323,共3页
Chinese Journal of Otorhinolaryngology
基金
国家自然科学基金资助项目 ( 39970 787)
关键词
耳蜗
毛细胞
钙通道
荧光染料
Cochlea
Hair cells, outer
Calcium channels
Fluorescent dyes
