摘要
本文介绍了生物发光法测定血清肌酸磷酸激酶(CK)的活性及其临床初步应用。实验中采用单一基质液,微量快速,简便灵敏,重复性好,专一性强,线性范围宽。批内批间变异系数分别为3.89％和8.72％,平均回收率98.65％。测定了50例正常人,19例急性心肌梗塞和3例进行性肌营养不良患者,血清CK活性分别为:142.22±52.40,1018.39±550.29,1661.98±686.27μmol/L/min。此方法优于一般的比色分析方法,为酶的测定提供了一个新的领域,有可能对疾病作出早期诊断,应用前景广阔。
This paper presented an assay method for creatine kinase in serum with bioluminenscence and It's clinical application. This assay method requied a least amount of reagents and sample size, was easy to perform, and also specific and reproducible. The coefficient of variation within assay is 3.8％ and 8.7％ between assay rate of recovery, 98.6％. The activities determined were 144.22±52.40, in healthy individual, 1018.39±550.29 in cardiac infarction, and 1661.98±689.27 in myopathy μ mol/L/min. This new method may be of value in the clinicap diagnosis and monitoring of certatn diseases.
出处
《军医进修学院学报》
CAS
1991年第3期236-238,共3页
Academic Journal of Pla Postgraduate Medical School
