摘要
应用形态计量学的方法，研究了日本血吸虫尾蚴减毒疫苗作用于小鼠后，宿主对再感染肝内虫卵肉芽肿细胞核形态计量学参数的变化。结果表明免疫组动物肝内形成的虫卵肉芽肿细胞数密度与面密度减小，与对照组有明显差异（Ｐ＜０００１）。细胞核的平均面积、平均周长、平均直径均改变明显（Ｐ＜００１）。而细胞核凹凸度改变不大（Ｐ＞００５）。揭示经疫苗作用后，宿主肝内虫卵肉芽肿反应下调现象明显，组织病理学改变减少。证明减毒疫苗产生保护性免疫力的机理。
A quantitative study was performed on egg granuloma cells in mice of experimental and control groups. In order to investigate the relationship between immunity and egg grunuloma lesions, numerical density and area density of grunulomus cells were measured using morphometric method. The results showed both numerical density and area density of the vaccination groups decreased obviously (p<0 001). The morphometric parameters showed significant differences (P<0 01) in area, perimeter and mean diameter of the nuclei as compared with those of the control groups. The form factor of nuclei in the experimented mice was also significantly different (p<0 05). Our data indicate that the granuloma reaction in host liver is markedly down regulated and that detoxicated vaccine may provide an immuno protection effect.
出处
《中国体视学与图像分析》
1997年第3期165-167,共3页
Chinese Journal of Stereology and Image Analysis
关键词
日本血吸虫病
形态计量学
减毒疫苗
虫卵肉芽肿细胞
schistosomiasis japonica
morphometric study, detoxicated vaccine, egg granuloma cell
