摘要
通过层析实验 ,比较了几种阴离子交换剂在不同温度、p H值、离子强度、进样流速、进样浓度下对人血清白蛋白的吸附与脱附性能 ,研究了它们对人血清白蛋白和卵清蛋白的分离能力 ,并考察了柱层析过程中吸附载体对重组人血清白蛋白和杂蛋白的分离选择性。实验结果表明 :牌号为DEAE Sepharose FF的载体不仅对重组人血清白蛋白具有良好的分离度 。
Adsorption and elution characteristics of human serum albumin (HSA) and recombinant HSA under different operation conditions, such as pH, ionic strength of buffer solution, flow rate and concentration of feed were investigated in fixed bed chromatography with three kinds of anion exchange gel beads as supports. Separation of HSA and ovalbumin in chromatography was studied. Selectivity between recombinant human serum albumin and hybrid proteins by anion exchange chromatography were discussed. It was experimentally shown that high resolution and recovery of recombinant HSA were obtained in fixed bed chromatography with DEAE Sepharose FF as support.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2002年第4期341-345,350,共6页
Journal of East China University of Science and Technology
基金
国家自然科学基金资助项目 ( 2 9976 0 14 )
