摘要
利用DNA重组技术 ,将杆状病毒极早期基因ie1启动子基因克隆至重组质粒pGEM Se39中 ,成功地构建了重组真核表达质粒pGEM IE1 Se39.
The baculovirus immediately early gene ie1 promoter was subcloned into recombinant plasmid pGEM-Se39 by using DNA recombinant technique. After transformation, the recombinant plasmid was isolated and digested with different endo-restriction enzymes. The result showed that the recombinant eukaryotic expression plasmid pGEM-IE1-Se39 containing an ie1 promotor was constructed successfully.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2002年第3期345-347,352,共4页
Journal of Central China Normal University：Natural Sciences
基金
国家自然科学基金资助项目 ( 30 170 0 11)
国家教育部高等学校骨干教师资助计划 (GG -180 - 10 5 11- 10 10 ) .
