摘要
目的探讨补肾中药龟板对局灶性脑缺血后脑组织3种NOS亚型的作用。方法采用大脑中动脉线栓法造成大鼠局灶性脑缺血模型,应用免疫组织化学技术检测脑组织3种亚型NOS(nNOS、iNOS、eNOS)的表达,观察龟板对脑缺血后3种亚型NOS的作用。结果龟板组缺血侧皮层和尾壳核nNOS、iNOS表达显著低于模型对照组(P<0.01),而eNOS表达显著高于模型对照组(P<0.01)。结论中药龟板能部分下调脑缺血所致nNOS、iNOS的异常表达,上调eNOS表达,这可能是其治疗缺血性脑血管疾病的机理之一。
Objective: To explore the effect of Plastrum Testudinis on the three subtypes of NOS (nNOS,iNOS,eNOS) expression in rats with focal cerebral ischemia. Methods:Rat model of focal cerebral ischemia was set up by inserting nylon thread into the internal carotid artery . The expressions of nNOS, iNOS and eNOS was detected by immunohistochemical method to observe the effects of Plastrum Testudinis. Results: Expressions of nNOS and iNOS in cerebral cortex and caudate-putamen of Plastrum Testudinis treatment group were significantly lower than those of model control group (P < 0.01); eNOS expression in cerebral cortex and caudate-putamen in Plastrum Testudinis treatment group was markedly higher than that of model control group (P < 0.01). Conclusion: Plastrum Testudinis can decrease nNOS and iNOS expression and increase eNOS expression and this may be one of its therapeutic mechanisms for cerebral ischemia.
出处
《中药新药与临床药理》
CAS
CSCD
2002年第5期278-281,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金资助项目(编号:39970914)。
关键词
动物模型
龟甲
药理学
局灶性脑缺血
中药疗法
神经保护作用
Carapax et plastrum testudinis/pharmacology
@ Focal cerebral ischemia/TCD therapy
@nNOS/analysis
@iNOS/analysis
@eNOS/analysis
Disease models, animal
