摘要
目的 探讨皮下局部注射血管内皮细胞生长因子 (VascularEndothelialGrowthFactor ,VEGF)对全厚随意型皮瓣成活以及皮瓣组织活性的影响。方法 选用 30只Wistar大鼠 ,随机分成三组 ,在其背部形成 3cm×7.5cm的全厚随意型皮瓣。术后即刻 :在其远蒂端 6 .5cm以及 5 .5cm处选择四个对称的注射位点 ,每一位点分别给予 10ng/ 10 0 μl的VEGF、0 .1%BSA、医用生理盐水各 10 0 μl。术后 6小时、2 4小时重复给药。观察 7天后将动物处死 ,计算皮瓣成活面积及观察皮瓣组织活性。结果 术后第三天皮瓣成活率 :VEGF组与其他两组间有显著性差异。术后第七天皮瓣成活率VEGF组与其他两组间有显著性差异。VEGF组远蒂端 7.5cm～ 4 .5cm范围内SDH含量高于其他两组。
Objective To determine the effects of vascular endothelial growth factor(VEGF)on survival of a full thickness random pattern,NcFarlane musculocutaneous flap in the rat.In addition,this study examined the effects of VEGF on the tissue viability of the distal of the flaps.Methods A 3cm× 7.5 cm full thickness dorsal flap with the pedicle remaining attached at the posterior end was elevated in 30 Wistar rats.The rats was randomised into three groups and received the following treatment:Group VEGF(n=10):subdermal VEGF injection into the flap(40ng/400ul) at 0,6,24h post flap elavation:Group BSA(n=10):subdermal 0.1 %BSA injection into the flap(400ul)at 0,6,24h post flap elavation:Group Normal saline:subdermal Normal saline injection into the flap(400ul) at 0,6,24h post flap elavation.Results After three days recovery,the area of flap survival was measured.There was swignificant difference between Group VEGF and the other groups.After seven days recovery,the area of flap survival was measured.There was significant diffcrcncc bctween Group VEGF and the other groups.Mean rate of cell survival and mean SDH of the distal of the flap in Group VEGF were significantly different from the other groups,but there was no significant difference among the three groups in the anterior flap.Conclusion These results support the notion that VEGF rescues tissue at risk of hyposic damage by inducing angiogenesis,and the use of growth factors such as VEGF holds promise as a method of increasing skin viability..
出处
《现代口腔医学杂志》
CAS
CSCD
2002年第5期397-400,共4页
Journal of Modern Stomatology
