摘要
通过检测植物生长激素研究不同茬高多花黑麦草(Lolium multiflorum)叶再生的机制。试验结果表明,遮光条件下,高茬和高茬断根的再生叶片生物量均显著高于低茬的再生叶片生物量(P<0.05)。光照试验结果表明,低茬在单次去叶时、高茬在多次去叶时均易提高总生物量系数(总生物量系数表示单位茬质量所支撑的再生过程中总有机物质量的变化,是用来评价非贮存有机物质参与叶片的再生能力,是不同茬高黑麦草再生能力强弱的指标)。通过检测光照条件下的叶片和伤流液中玉米素+玉米素核苷(Z+ZR)及脱落酸(ABA)含量,发现叶Z+ZR含量与总生物量系数相关密切,且叶Z+ZR含量受根系的直接调控。基于根系对叶片的影响,根系诱导的叶片细胞分裂素是影响不同茬高黑麦草再生的关键因素。
The regrowth mechanism of ryegrasses(Lolium multiflorum)with different stubble heights was investigated.Experiments with different photoperiod were conducted.Results showed that the plants in darkness with low stubble height had significant lower leaf regrowth biomass(P<0.05)compared with the plants with high stubble height and root clipping.Total biomass index which showed the variation of total organic substances during regrowth with single stubble was used to evaluate leaf regrowth with different stubble heights with the minimized influence of stubble stored organic substances.The plants with light,single clipping and low stubble height had higher total biomass index.The plants with light,frequent clipping and high stubble height also had higher total biomass index.Concentrations of zeatin and zeatin riboside(Z+ZR)and abscisic acid(ABA)in leaves and xylem sap from the plants with light were measured and showed that total biomass index had positive correlation with leaf Z+ZR concentrations which adjusted by roots.It can be concluded that root-induced leaf cytokinin was critical factor affecting ryegrass regrowth at different clipping heights
出处
《草业科学》
CAS
CSCD
北大核心
2014年第7期1310-1317,共8页
Pratacultural Science
基金
国家自然科学基金(U1304326)
河南省教育厅自然科学研究项目(2011A180012)